| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | De2f2; E2F transcription factor 2; E2F-2; Transcription factor E2F2;;E2F2 |
| WB Predicted band size | Calculated MW: 48 kDa ; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human E2F2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于E2F2抗体的3篇参考文献示例(注：文献信息为模拟示例，仅供参考)：
1. **文献名称**：E2F2 regulates cell cycle progression and apoptosis in cancer cells
**作者**：Smith A, et al.
**摘要**：本研究利用E2F2特异性抗体，通过ChIP-seq和Western blot分析，揭示了E2F2在调控G1/S期转换中的关键作用，并发现其过表达可诱导肿瘤细胞凋亡。
2. **文献名称**：Dysregulation of E2F2 in hepatocellular carcinoma: A biomarker study
**作者**：Zhang L, et al.
**摘要**：通过免疫组化(使用E2F2抗体)和qPCR，研究发现肝癌组织中E2F2表达显著上调，且与患者预后不良相关，提示其作为潜在治疗靶点的可能性。
3. **文献名称**：E2F2 antibody-based inhibition suppresses glioblastoma growth in vivo
**作者**：Brown K, et al.
**摘要**：该研究开发了一种高特异性E2F2单克隆抗体，在小鼠胶质母细胞瘤模型中证实其可通过阻断E2F2-DNA结合抑制肿瘤增殖，为靶向治疗提供新策略。
如需具体文献，建议通过PubMed或Google Scholar检索关键词“E2F2 antibody”获取最新研究。
The E2F2 antibody is a crucial tool in studying the E2F family of transcription factors, which play pivotal roles in cell cycle regulation, apoptosis, and differentiation. E2F2. a member of the E2F family, primarily functions as a transcriptional activator that drives the expression of genes essential for G1/S phase transition, including cyclin E and CDK2. It binds to target promoters through conserved DNA-binding domains and collaborates with DP proteins to regulate cell proliferation. E2F2 is particularly associated with the activation of quiescent cells into the cell cycle, and its dysregulation has been implicated in cancer development, where overexpression may lead to uncontrolled proliferation or genomic instability.
E2F2 antibodies are widely used in research to detect and quantify E2F2 protein levels in various experimental models, such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry. These antibodies help elucidate E2F2's spatial-temporal expression patterns, post-translational modifications, and interactions with regulatory proteins like retinoblastoma (Rb) family members. Studies using E2F2-deficient mice have highlighted its role in hematopoiesis and lymphocyte function, further underscoring its biological significance. Commercially available E2F2 antibodies are typically validated for specificity against conserved epitopes, though cross-reactivity with other E2F family members (e.g., E2F1 or E2F3) remains a consideration. Their applications extend to cancer research, where E2F2 expression profiles are analyzed in tumor tissues to assess prognostic or therapeutic relevance.
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