Rabbit Monoclonal proCaspase10 Antibody

The proCaspase-10 antibody is a crucial tool for studying the regulation and function of Caspase-10. a member of the cysteine-aspartic protease (caspase) family involved in apoptosis and inflammation. Caspase-10 exists as an inactive zymogen (proenzyme) that requires proteolytic cleavage for activation. It plays a key role in the extrinsic apoptotic pathway, initiated by death receptors like FAS and TRAIL, where it processes downstream effector caspases (e.g., Caspase-3/7) to execute cell death. ProCaspase-10 antibodies are designed to detect the full-length, unprocessed form (∼55 kDa) of the protein, aiding in studies of its expression, activation kinetics, and dysregulation in diseases. These antibodies often target epitopes in the N-terminal prodomain or the large catalytic subunit. Research using proCaspase-10 antibodies has linked its aberrant expression or mutations to autoimmune disorders (e.g., ALPS), cancer resistance to apoptosis, and neurodegenerative conditions. They are widely employed in techniques like Western blotting, immunohistochemistry, and flow cytometry to explore Caspase-10's role in cell death signaling, immune response modulation, and therapeutic targeting. Validating antibody specificity is critical, as cross-reactivity with homologous caspases (e.g., Caspase-8) may occur due to structural similarities in their death effector domains (DEDs).