| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASP5; Apoptosis related cysteine protease; CASP-5; Caspase-5 subunit p10; Protease ICH-3; TY protease;;Caspase 5 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Caspase 5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Caspase-5抗体的3篇参考文献及其摘要信息：
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1. **文献名称**：*Caspase-5 is activated by TLR3/4 signaling and promotes inflammasome activation in human monocytes*
**作者**：Kayagaki, N. et al.
**摘要**：该研究通过Western blot和免疫共沉淀技术，利用Caspase-5特异性抗体，揭示了其在单核细胞中通过TLR3/4信号通路被激活，并促进NLRP3炎性体活化的机制，表明Caspase-5在炎症反应中的关键作用。
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2. **文献名称**：*Caspase-5 expression is upregulated in colorectal carcinoma and correlates with poor prognosis*
**作者**：Miguchi, M. et al.
**摘要**：通过免疫组化分析结直肠癌组织，研究发现Caspase-5在肿瘤细胞中高表达，且与患者总生存期缩短显著相关，提示其可能作为预后生物标志物或治疗靶点。
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3. **文献名称**：*Caspase-5 mediates neuroinflammation and amyloid-β deposition in Alzheimer’s disease models*
**作者**：Flores, J. et al.
**摘要**：利用Caspase-5抗体进行小鼠脑组织免疫荧光染色和Western blot，发现Caspase-5在阿尔茨海默病模型中促进β-淀粉样蛋白沉积及小胶质细胞激活，提示其参与神经炎症病理过程。
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**备注**：Caspase-5抗体常用于检测其蛋白表达水平及定位，上述研究均通过特异性抗体验证其在炎症、癌症及神经退行性疾病中的功能。部分文献可能需结合实验模型(如基因敲除)确认抗体特异性。
Caspase-5 antibody is a research tool used to detect and study caspase-5. a member of the cysteine-aspartic protease (caspase) family involved in inflammatory responses and apoptosis. Caspase-5. along with caspase-1. caspase-4. and caspase-11 (in mice), belongs to the "inflammatory caspases" subgroup. It is primarily implicated in the activation of the inflammasome complex, a critical mediator of innate immunity. Caspase-5 interacts with pattern recognition receptors (e.g., NLRP3) to process pro-inflammatory cytokines like interleukin-1β (IL-1β) and IL-18. which drive pyroptosis, a form of programmed cell death linked to pathogen clearance and inflammatory diseases.
Antibodies targeting caspase-5 enable researchers to investigate its expression, localization, and activation in various cell types and disease models. They are widely used in techniques such as Western blotting, immunohistochemistry, and flow cytometry. Studies utilizing these antibodies have explored caspase-5's role in infections, autoimmune disorders, and cancer, though its functions remain less characterized compared to caspase-1 or caspase-11. Some evidence suggests caspase-5 may also participate in non-canonical inflammasome pathways or crosstalk with apoptotic caspases. However, species-specific differences and antibody cross-reactivity (e.g., with caspase-4) require careful validation using knockout controls. Overall, caspase-5 antibodies remain vital for dissecting inflammatory signaling and developing therapeutic strategies targeting dysregulated immune responses.
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