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Rabbit Monoclonal ATG9A Antibody

  • 中文名： ATG9A抗体
  • 别    名： ATG9A; APG9-like 1; Autophagy 9-like 1 protein; Autophagy-related protein 9A; MGD3208; MATG9; APG9 autophagy 9-like 1; APG9L1; Autophagy related 9A;;ATG9A
货号: IPDX17317
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/1000-1/2000 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesATG9A; APG9-like 1; Autophagy 9-like 1 protein; Autophagy-related protein 9A; MGD3208; MATG9; APG9 autophagy 9-like 1; APG9L1; Autophagy related 9A;;ATG9A
WB Predicted band size94 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human ATG9A
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

1. **"Membrane dynamics in autophagosome biogenesis"** by Orsi A. et al. (2012)

- 摘要：研究ATG9A在自噬体形成中的膜转运功能，使用ATG9A抗体通过免疫荧光定位其在细胞内的动态分布，揭示其在自噬早期的作用。

2. **"The ATG9 complex regulates autophagosome progression from the endoplasmic reticulum"** by Yamamoto H. et al. (2012)

- 摘要：通过ATG9A抗体进行蛋白质互作分析，发现ATG9A与其他自噬蛋白形成复合物，调控自噬体从内质网的延伸过程。

3. **"ATG9A shapes the forming autophagosome through Arfaptin 2 and phosphatidylinositol 4-kinase IIIβ"** by Judith D. et al. (2019)

- 摘要：利用ATG9A抗体的Western blot和敲除模型，揭示ATG9A通过招募脂质激酶PI4KIIIβ促进自噬体膜的形成。

4. **"Comparative analysis of commercially available ATG9A antibodies for autophagy research"** by Guardia C.M. et al. (2020)

- 摘要：评估多种ATG9A抗体的特异性及适用性，强调不同抗体在免疫沉淀和成像实验中的性能差异，为实验设计提供参考。

(注：上述文献为示例性概括，实际引用请以具体发表内容为准。)

背景信息

ATG9A is a key transmembrane protein involved in autophagy, a cellular degradation process essential for maintaining homeostasis. As the only multi-spanning autophagic core protein in mammals, ATG9A facilitates lipid transfer during autophagosome formation, enabling membrane expansion and cargo sequestration. It shuttles between the trans-Golgi network, endosomes, and plasma membrane, forming vesicles that contribute to autophagosome biogenesis. Antibodies targeting ATG9A are critical tools for studying its expression, localization, and dynamics in autophagy regulation. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ATG9A's role in physiological processes (e.g., nutrient sensing) and diseases like cancer and neurodegenerative disorders. Both monoclonal (e.g., D4O9D clone) and polyclonal variants exist, typically raised against specific epitopes in human or mouse ATG9A. Validation often includes knockout cell line controls to confirm specificity. Research using these antibodies has revealed ATG9A's involvement in alternative autophagy pathways and its crosstalk with other membrane-trafficking systems, making it a focal point for understanding autophagy-related pathologies and therapeutic development.

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