首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰岛素样生长因子2(IGF-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF2含量。IGF2即胰岛素样生长因子 2，在胚胎发育和生长调节中至关重要。其异常表达与肿瘤发生发展紧密相关，常通过激活下游信号通路促进细胞增殖、抑制凋亡。目前研究围绕其信号机制，有望开发靶向药物，为癌症等疾病治疗提供新思路。试剂盒检测范围为78 pg/mL-5000 pg/mL，支持科研人员探究生长发育调控机制、代谢相关疾病模型（如糖尿病、肥胖）的分子机理，以及肿瘤微环境中IGF - 2的表达模式分析，适用于实验室常规批量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Igf2 ELISA Kit; Igf-2 ELISA Kit; Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Multiplication-stimulating activity ELISA Kit; MSA ELISA Kit; Multiplication-stimulating polypeptide) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写：IGF2
Uniprot No.：P01346
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 85-104 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 85-89 | |||||
回收率：
| The recovery of rat IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 101-109 | ||||
| EDTA plasma (n=4) | 97 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.4022.4592.4312.32925002.1392.1452.1422.04012501.6741.6621.6681.5666251.0851.0981.0920.9903120.5380.5480.5430.4411560.3140.3260.3200.218780.1960.1860.1910.08900.1010.1030.102 | ||||||
货期：3-5 working days
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