| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIT1 |
| Uniprot No | Q9Y3P8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 62-196aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMHLSQWTRGRSRSHPGQGRSGESVEEVPLY GNLHYLQTGRLSQDPEPDQQDPTLGGPARAAEEVMCYTSLQLRPPQGRIP GPGTPVKYSEVVLDSEPKSQASGPEPELYASVCAQTRRARASFPDQAYAN SQPAAS |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIT1重组蛋白的3篇文献示例(内容基于模拟数据，建议通过学术数据库验证具体信息)：
1. **文献名称**：*Structural insights into the autoinhibition mechanism of SIT1 protein*
**作者**：Gilbert, R. et al.
**摘要**：通过X射线晶体学解析SIT1重组蛋白的晶体结构，发现其N端结构域通过分子内相互作用抑制C端配体结合区域，揭示了SIT1自抑制的分子机制。
2. **文献名称**：*SIT1 interacts with TCR signaling components to modulate T cell activation*
**作者**：Wang, L. et al.
**摘要**：利用重组SIT1蛋白进行体外结合实验，证明其与T细胞受体(TCR)复合物中的CD3ε链直接结合，并调控T细胞活化信号通路的强度。
3. **文献名称**：*Recombinant SIT1 expression in E. coli and its role in antigen presentation*
**作者**：Zhang, Y. et al.
**摘要**：优化SIT1重组蛋白在大肠杆菌中的可溶性表达方案，并验证其与MHC-II分子协同作用，促进抗原呈递细胞向T细胞传递信号的生物学功能。
(注：若需真实文献，建议在PubMed或Google Scholar中检索关键词“SIT1 recombinant protein”或“SIT1 signaling”。)
SIT1 (signaling threshold-regulating transmembrane protein 1) is a cell surface protein primarily expressed in immune cells, particularly T lymphocytes. It belongs to the immunoglobulin superfamily and functions as a negative regulator of T-cell receptor (TCR) signaling. Discovered in the early 2000s, SIT1 interacts with TCR complex components and adaptor molecules to modulate signal transduction thresholds, thereby preventing excessive immune activation. Its structure includes extracellular Ig-like domains, a transmembrane region, and cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit phosphatases to dampen signaling cascades.
Recombinant SIT1 protein is produced using expression systems like mammalian cells or *E. coli*, engineered to include epitope tags for purification and detection. Researchers utilize it to study immune regulation mechanisms, particularly in autoimmune diseases, allergies, and cancer immunotherapy. In vitro studies demonstrate that soluble SIT1 protein can competitively inhibit membrane-bound SIT1 function, enhancing T-cell responses. This property has spurred interest in developing SIT1-based biologics to modulate immune activity. Recent investigations also explore its role in viral infections and cross-talk with checkpoint inhibitors like PD-1. While clinical applications remain exploratory, SIT1 recombinant proteins serve as vital tools for deciphering immune tolerance dynamics and designing targeted immunotherapies. Its dual role as both a membrane-bound modulator and soluble signaling mediator makes it a unique subject in adaptive immunity research.
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