| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PEBP1; HCNP; PBP; PEBP; RKIP;;PEBP1 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PEBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PBP(青霉素结合蛋白)抗体的3篇参考文献示例，涵盖不同研究方向：
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1. **文献名称**：*Development of Monoclonal Antibodies Against PBP2a in Methicillin-Resistant Staphylococcus aureus*
**作者**：Smith A, et al.
**摘要**：研究团队开发了针对MRSA中PBP2a蛋白的高特异性单克隆抗体，验证了其在免疫印迹和快速检测试纸中的应用，显著提升了耐药菌的临床诊断效率。
2. **文献名称**：*Antibody-Based Detection of Altered Penicillin-Binding Proteins in Streptococcus pneumoniae*
**作者**：Johnson B, et al.
**摘要**：通过制备多克隆抗体，分析了肺炎链球菌中PBP1A和PBP2B的变异体，揭示了抗体在识别β-内酰胺耐药相关PBP突变中的潜力。
3. **文献名称**：*Novel Immunoassay for Quantifying PBP Levels in Gram-Negative Bacteria*
**作者**：Lee C, et al.
**摘要**：建立了一种基于抗体的ELISA方法，用于定量大肠杆菌等革兰氏阴性菌中PBP3的表达水平，为研究抗生素作用机制提供了工具。
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**注**：以上文献为示例性内容，实际研究请通过PubMed、Web of Science等平台检索最新论文。如需具体文献，可提供更详细的研究方向(如特定病原体或应用场景)。
Penicillin-binding protein (PBP) antibodies are immunological tools used to study PBPs, a group of bacterial enzymes critical for peptidoglycan synthesis during cell wall formation. PBPs bind β-lactam antibiotics (e.g., penicillin) via their active sites, a mechanism exploited by these drugs to inhibit cell wall assembly and kill bacteria. However, antibiotic resistance often arises from PBP modifications, such as mutations or acquisition of alternative PBPs (e.g., PBP2a in methicillin-resistant *Staphylococcus aureus* [MRSA]), which reduce drug binding affinity.
PBP antibodies are developed to detect and characterize these proteins in bacterial pathogens. They aid in understanding resistance mechanisms, strain typing, and epidemiological surveillance. For example, anti-PBP2a antibodies are used in rapid diagnostic assays to confirm MRSA infections by identifying the presence of the *mecA*-encoded PBP2a. Similarly, antibodies against PBPs in *Streptococcus pneumoniae* help track vaccine escape strains with altered PBPs.
These antibodies are typically generated through immunization with recombinant PBP fragments or synthetic peptides. They enable techniques like Western blotting, ELISA, and immunofluorescence to study PBP expression, localization, and interaction with antibiotics. Recent research also explores PBP-targeting monoclonal antibodies as potential therapeutics to enhance β-lactam efficacy against resistant strains. Overall, PBP antibodies serve as vital reagents in both basic microbiology and clinical diagnostics, bridging antibiotic discovery and resistance management.
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