| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIRT3 |
| Uniprot No | Q9NTG7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 118-399aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSDKGKLSLQDVAELIRARACQRVVVMVGA GISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFF HNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERV SGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGV VKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRS SVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDL VQRETGKLDGPDK |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIRT3重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation*
**作者**：Ahn, B.H., et al. (2008)
**摘要**：该研究通过体外实验证明，重组SIRT3蛋白能够去乙酰化线粒体中的长链酰基辅酶A脱氢酶(LCAD)，增强其活性，从而调控脂肪酸氧化和能量代谢，揭示了SIRT3在代谢稳态中的关键作用。
2. **文献名称**：*Mammalian Sir2 Homolog SIRT3 Regulates Global Mitochondrial Lysine Acetylation*
**作者**：Lombard, D.B., et al. (2007)
**摘要**：研究发现重组SIRT3蛋白具有NAD⁺依赖的脱乙酰酶活性，并系统鉴定了其在线粒体中的底物，表明SIRT3通过调控蛋白乙酰化水平维持线粒体功能及氧化代谢平衡。
3. **文献名称**：*SIRT3 Deacetylates ATP Synthase F1 Complex Proteins in Response to Nutrient- and Exercise-Induced Stress*
**作者**：Hebert, A.S., et al. (2013)
**摘要**：利用重组SIRT3蛋白进行体外实验，发现其在能量应激条件下(如饥饿或运动)通过去乙酰化ATP合酶亚基，调节线粒体ATP生成，强调了SIRT3在代谢适应中的动态调控作用。
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以上研究均通过重组SIRT3蛋白揭示了其在代谢、氧化应激和疾病中的分子机制，为靶向SIRT3的治疗策略提供了理论基础。如需具体文献来源，建议通过PubMed或Sci-Hub检索标题获取全文。
SIRT3 (sirtuin 3) is a NAD⁺-dependent deacetylase belonging to the sirtuin family, primarily localized in mitochondria. It plays a pivotal role in regulating energy metabolism, antioxidant defense, and mitochondrial homeostasis by modifying acetylation states of target proteins. SIRT3 activates key metabolic enzymes (e.g., ACSS2. SOD2) and modulates pathways critical for fatty acid oxidation, oxidative phosphorylation, and reactive oxygen species (ROS) scavenging. Its activity is linked to lifespan extension, stress resistance, and metabolic adaptation in response to caloric restriction.
Recombinant SIRT3 protein is engineered through heterologous expression systems (e.g., E. coli or mammalian cells) for in vitro studies. The purified protein retains deacetylase activity and is widely used to investigate molecular mechanisms of mitochondrial regulation, aging-related diseases, and metabolic disorders. Researchers employ it to screen activators/inhibitors, study post-translational modifications, or validate interactions with substrates. Its applications extend to modeling neurodegenerative, cardiovascular, and cancer-related pathologies where SIRT3 dysregulation is implicated. Current studies focus on harnessing SIRT3's therapeutic potential, particularly in metabolic syndrome and age-associated decline. Recombinant SIRT3 serves as a vital tool for decoding mitochondrial biology and developing precision therapies.
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