首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子α干扰素(IFN-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNA1含量。IFNA1 即α1型干扰素，其具有抗病毒、免疫调节等多种作用。在研究机制方面，它可与细胞表面受体结合，激活下游信号通路，诱导多种抗病毒蛋白产生，发挥抑制病毒复制和调节机体免疫反应等功能，是抗病毒和免疫相关研究的重要靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于生命科学研究中病毒侵染模型构建、免疫调节机制解析以及疫苗/抗病毒药物研发等场景，特别适用于兔科动物模型中天然免疫反应的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFN-alpha，interferon alpha
缩写：IFN-α
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of rabbit IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 94 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.8532.6812.7672.6752002.2242.3312.2782.1861001.4981.5411.5201.428500.8760.8870.8820.790250.4430.4320.4380.34612.50.2390.2270.2330.1416.250.1500.1460.1480.05600.0920.0910.092 | ||||||
货期：3-5 working days
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