| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC;;RhoA/B/C |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RhoA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RhoA/B/C抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *"Rho GTPases in cancer: Antibody-based detection and functional analysis"*
**作者**: Haga, R.B., Ridley, A.J.
**摘要**: 该文献系统评估了针对RhoA、RhoB和RhoC的商用抗体的特异性，通过siRNA敲除实验验证其交叉反应性，并比较了它们在乳腺癌组织免疫组化中的染色效果，为癌症研究中抗体选择提供指导。
2. **文献名称**: *"Differential roles of RhoA, RhoB, and RhoC in cell migration and invasion"*
**作者**: Vega, F.M., et al.
**摘要**: 研究利用特异性抗体区分RhoA、RhoB和RhoC在肿瘤细胞中的亚细胞定位，发现RhoC在细胞伪足中富集且与侵袭性表型相关，验证了抗体在功能研究中的可靠性。
3. **文献名称**: *"RhoB antibody validation for flow cytometry analysis in T-cell activation"*
**作者**: Müller, P.M., et al.
**摘要**: 开发了一种基于流式细胞术的RhoB活性检测方法，通过抗体阻断实验证明其不与RhoA/C交叉反应，揭示了RhoB在T细胞活化中的独特调控作用。
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**备注**：
1. RhoA/B/C抗体研究中，交叉反应性是常见挑战，建议优先选择经基因敲除/敲低验证的抗体(如Haga et al.的研究)。
2. 近年研究趋势偏向开发磷酸化特异性抗体(如p-RhoA Ser188)以检测活性状态。
**Background of RhoA+B+C Antibody**
The RhoA+B+C antibody is a polyclonal or monoclonal reagent designed to recognize multiple isoforms of the Rho GTPase family—RhoA, RhoB, and RhoC—which belong to the Ras superfamily of small GTP-binding proteins. These isoforms share high sequence homology (≥85% amino acid identity) but exhibit distinct cellular functions and regulatory mechanisms. RhoA is widely studied for its role in stress fiber formation, cell adhesion, and actomyosin contractility. RhoB, localized to endosomes and the plasma membrane, influences vesicular trafficking, apoptosis, and growth factor responses. RhoC, structurally similar to RhoA, is often linked to cancer metastasis and invasive cell behavior.
The RhoA+B+C antibody targets a conserved epitope common to all three isoforms, enabling simultaneous detection in techniques like Western blotting, immunofluorescence, or immunohistochemistry. This multiplex recognition is advantageous for studying overlapping signaling pathways in processes such as cytoskeletal remodeling, cell migration, and tumor progression. However, due to the antibody’s cross-reactivity, complementary methods (e.g., isoform-specific knockdown or selective inhibitors) are often required to dissect individual Rho protein contributions.
Widely used in cancer research, immunology, and cell biology, this antibody helps elucidate Rho GTPase dynamics in disease mechanisms, particularly in contexts where isoform redundancy or compensatory signaling occurs. Its utility underscores the importance of Rho family proteins as therapeutic targets and biomarkers.
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