| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H1b; Histone H1s-4;;p-Histone H1.4 (T17) |
| WB Predicted band size | Calculated MW: 22 kDa ; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H1.4 around the phosphorylation site of T17 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-Histone H1.4 (Thr17)抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "Phosphorylation of Histone H1.4 at Thr17 regulates mitotic chromatin dynamics"
**作者**: Goto, H. et al.
**摘要**: 研究揭示了Histone H1.4在Thr17位点的磷酸化通过调控染色质凝聚促进有丝分裂进程，并证实该抗体特异性识别此修饰位点。
2. **文献名称**: "DNA damage-induced phosphorylation of H1.4 Thr17 modulates apoptosis signaling"
**作者**: Kim, S.J. & Lee, J.
**摘要**: 发现DNA损伤后，H1.4的T17磷酸化通过激活p53通路增强细胞凋亡，研究使用该抗体验证了其在电离辐射模型中的修饰动态。
3. **文献名称**: "H1.4 Thr17 phospho-specific antibody as a biomarker for cancer cell proliferation"
**作者**: Smith, R. et al.
**摘要**: 评估Phospho-H1.4(T17)抗体在多种癌症组织中的表达水平，发现其与肿瘤细胞增殖活性显著相关，提示其临床诊断潜力。
4. **文献名称**: "Development and validation of a phospho-specific antibody targeting Histone H1.4 Thr17"
**作者**: Rodriguez, M. et al.
**摘要**: 描述了该抗体的开发流程，通过质谱和突变实验验证其特异性，并应用于染色质免疫沉淀(ChIP)分析细胞周期相关基因调控。
注：以上文献为示例，实际文献需通过PubMed或Google Scholar等平台检索确认。
The Phospho-Histone H1.4 (T17) antibody is a specialized tool used to detect the phosphorylation of histone H1.4 at threonine 17. a post-translational modification linked to chromatin dynamics and cellular processes. Histone H1.4. a linker histone, stabilizes higher-order chromatin structure and regulates gene expression by binding to nucleosomes. Phosphorylation of H1.4 at T17 is associated with chromatin relaxation during the cell cycle, particularly in mitosis or DNA damage response, facilitating access to repair machinery or transcriptional activation. This modification may also play a role in apoptosis and cellular differentiation.
The antibody is commonly applied in techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP) to study H1.4 phosphorylation dynamics in models of cancer, stem cell biology, or genotoxic stress. Researchers use it to explore how T17 phosphorylation correlates with cell cycle progression (e.g., G2/M transition), DNA damage pathways (e.g., ATM/ATR signaling), or epigenetic reprogramming. Specificity validation often includes knockout/knockdown controls or phosphatase treatment to confirm phospho-dependence.
Understanding H1.4(T17) phosphorylation provides insights into chromatin remodeling mechanisms and disease-related dysregulation, making this antibody valuable for basic and translational research in epigenetics and oncology.
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