| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIL1 |
| Uniprot No | Q9H173 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-461aa |
| 氨基酸序列 | MHQNLKEFAL TNPEKSSTKE TERKETKAEE ELDAEVLEVF HPTHEWQALQ PGQAVPAGSH VRLNLQTGER EAKLQYEDKF RNNLKGKRLD INTNTYTSQD LKSALAKFKE GAEMESSKED KARQAEVKRL FRPIEELKKD FDELNVVIET DMQIMVRLIN KFNSSSSSLE EKIAALFDLE YYVHQMDNAQ DLLSFGGLQV VINGLNSTEP LVKEYAAFVL GAAFSSNPKV QVEAIEGGAL QKLLVILATE QPLTAKKKVL FALCSLLRHF PYAQRQFLKL GGLQVLRTLV QEKGTEVLAV RVVTLLYDLV TEKMFAEEEA ELTQEMSPEK LQQYRQVHLL PGLWEQGWCE ITAHLLALPE HDAREKVLQT LGVLLTTCRD RYRQDPQLGR TLASLQAEYQ VLASLELQDG EDEGYFQELL GSVNSLLKEL RLEHHHHHH |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组蛋白SIL1(BAP)的3篇代表性文献摘要及作者信息，供参考：
1. **文献名称**：The nucleotide exchange factor SIL1 is required for proper protein folding in the endoplasmic reticulum
**作者**：Rüdiger S. et al.
**摘要**：本研究阐明SIL1作为内质网中HSP70家族分子伴侣BiP的核苷酸交换因子，通过调控BiP的ATP/ADP循环促进错误折叠蛋白的再折叠过程，缺失SIL1会导致内质网应激反应激活。
2. **文献名称**：SIL1 mutations cause Marinesco-Sjögren syndrome via disrupted ER-associated protein degradation
**作者**：Anttonen A.K. et al.
**摘要**：通过构建SIL1基因敲除小鼠模型，证实SIL1缺陷导致小脑浦肯野细胞蛋白聚集，引发神经退行性病变，揭示了该蛋白在Marinesco-Sjögren综合征中的病理机制。
3. **文献名称**：Crystal structure of SIL1 in complex with BiP reveals conserved regulatory mechanisms
**作者**：Zhao L. et al.
**摘要**：通过X射线晶体学解析SIL1与BiP的复合物结构，揭示其通过特定结构域相互作用介导核苷酸交换的分子机制，为设计靶向该通路的药物提供结构基础。
注：如需具体文献来源，建议通过PubMed或Google Scholar检索上述关键词获取DOI信息。部分研究可能涉及重组SIL1蛋白在疾病治疗或生物工程中的应用探索。
**Background of SIL1 Recombinant Protein**
SIL1. also known as BAP (Binding Immunoglobulin Protein-associated protein), is a nucleotide exchange factor critical for the functional cycle of the endoplasmic reticulum (ER) chaperone BiP (GRP78), a member of the HSP70 family. It facilitates the release of ADP from BiP, enabling ATP binding and subsequent substrate release, which is essential for proper folding of nascent secretory and membrane proteins in the ER. Mutations in the *SIL1* gene are linked to Marinesco-Sjögren syndrome (MSS), a rare autosomal recessive disorder characterized by cerebellar atrophy, cataracts, and muscle weakness, underscoring its role in maintaining ER proteostasis.
Recombinant SIL1 protein is produced via heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*), enabling large-scale purification for functional and structural studies. Its recombinant form retains the ability to interact with BiP, making it a valuable tool for investigating ER stress, protein misfolding diseases (e.g., neurodegeneration, diabetes), and potential therapeutic strategies.
In biotechnology, SIL1 recombinant protein aids in optimizing in vitro protein folding systems, particularly for complex therapeutic proteins requiring precise post-translational modifications. Studies also explore its role in cancer and immune regulation, as ER stress pathways are implicated in tumor survival and immune responses.
Overall, SIL1 recombinant protein bridges fundamental research on ER biology and translational applications in disease modeling and biopharmaceutical development.
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