| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKB;RAC;PRKBA;PKB-ALPHA;RAC-ALPHA; AKT1 ; AKT2; AKT3; Akt1/2/3; pan Akt;;AKT |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human AKT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于AKT1/2/3抗体的3篇参考文献示例(注：文献名称与作者为虚构示例，实际文献需通过数据库查询获取)：
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1. **文献名称**：*"Specificity and application of AKT isoform antibodies in cancer cell lines"*
**作者**：Johnson R, et al.
**摘要**：本研究系统评估了多种AKT1、AKT2和AKT3抗体在多种癌细胞系中的特异性。通过siRNA敲除实验验证抗体选择性，发现部分抗体存在亚型交叉反应，推荐在Western blot中使用特定克隆号抗体以确保准确性。
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2. **文献名称**：*"Phospho-specific AKT antibodies reveal differential activation in signaling pathways"*
**作者**：Chen L, et al.
**摘要**：开发并验证了针对AKT1/2/3磷酸化位点(Ser473)的抗体，证明其在流式细胞术和免疫荧光中的适用性。研究发现不同细胞类型中AKT亚型的激活模式差异，提示需结合亚型特异性抗体进行功能研究。
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3. **文献名称**：*"Comparative analysis of AKT3 antibody performance in neurological versus non-neurological tissues"*
**作者**：Martinez S, et al.
**摘要**：比较了5种商用AKT3抗体在脑组织与肿瘤组织中的表现，发现部分抗体在神经组织中非特异性结合较高，建议在使用前通过免疫沉淀验证抗体可靠性，尤其在研究AKT3与神经疾病关联时。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“AKT1 antibody validation”“AKT isoform-specific antibody”等获取近期高质量研究。
**Background of AKT1/2/3 Antibodies**
The AKT family, comprising AKT1 (PKBα), AKT2 (PKBβ), and AKT3 (PKBγ), are serine/threonine kinases central to the PI3K-AKT-mTOR signaling pathway. These isoforms share structural homology, including a pleckstrin homology (PH) domain, a catalytic kinase domain, and a regulatory hydrophobic motif. They regulate diverse cellular processes such as survival, proliferation, metabolism, and apoptosis. AKT1 is ubiquitously expressed and linked to cell growth, while AKT2 is critical in insulin signaling and glucose metabolism. AKT3. predominantly found in the brain, influences neurological development. Dysregulation of AKT activity, often due to mutations or overexpression, is implicated in cancers, diabetes, and neurological disorders.
Antibodies targeting AKT1/2/3 are essential tools for studying their expression, activation, and localization. These antibodies detect total AKT or phosphorylated forms (e.g., at Thr308/Ser473 for AKT1), reflecting kinase activation. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate AKT’s role in disease mechanisms or therapeutic responses. Isoform-specific antibodies are crucial due to functional differences, despite high sequence similarity. For instance, AKT2-selective antibodies help dissect metabolic signaling, while AKT3-specific ones explore neural pathways. Additionally, AKT antibodies aid in validating drug targets, such as PI3K-AKT inhibitors in oncology. Their specificity and reliability are vital for accurate research and diagnostic applications, making them indispensable in both basic and translational studies.
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