| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CKBBP1; Rbx2; RNF7; ROC2; SAG;;RNF7 |
| WB Predicted band size | 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RNF7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于RNF7抗体的参考文献及其摘要概括：
1. **文献名称**：*RNF7 regulates VHL-mediated hypoxia responses through ubiquitin-dependent degradation*
**作者**：Li Z, Wang D, Na X, et al.
**摘要**：该研究利用RNF7抗体验证RNF7在泛素-蛋白酶体系统中对VHL蛋白的调控作用，揭示其通过降解VHL促进缺氧诱导因子(HIF)信号通路激活的机制，与肾癌进展相关。
2. **文献名称**：*RNF7 as a potential biomarker in hepatocellular carcinoma: Overexpression correlates with poor prognosis*
**作者**：Chen L, Liu J, Zhang Y.
**摘要**：通过免疫组化(使用RNF7特异性抗体)发现RNF7在肝癌组织中高表达，且与患者生存率负相关，提示其可能作为肝癌预后标志物及治疗靶点。
3. **文献名称**：*RNF7 interacts with p53 and modulates its stability in response to DNA damage*
**作者**：Kim H, Park JH, Lee S.
**摘要**：研究通过免疫共沉淀(RNF7抗体)证实RNF7与p53直接结合，调控DNA损伤条件下p53的泛素化及稳定性，影响细胞凋亡和周期阻滞。
4. **文献名称**：*Development and validation of a monoclonal antibody against human RNF7 for functional studies*
**作者**：Wang Q, Li M, Zhou R.
**摘要**：报道一种高特异性抗人RNF7单克隆抗体的开发，验证其在Western blot、免疫荧光中的应用，并用于研究RNF7在泛素化复合体CRL5中的组装功能。
以上文献涵盖RNF7在分子机制、疾病标志物及抗体开发中的应用。如需具体期刊信息或PMID，可进一步补充。
RNF7 (Ring Finger Protein 7), also known as ROC2 or SAG, is a member of the RING finger protein family that functions as a critical component of the E3 ubiquitin ligase complex. It interacts with Cullin 5 (Cul5) to form the Cullin-RING ligase (CRL) complex, which plays a central role in ubiquitination-mediated protein degradation via the proteasome system. RNF7 is involved in regulating diverse cellular processes, including oxidative stress response, cell cycle progression, DNA repair, and apoptosis. Its dysregulation has been implicated in cancer, neurodegenerative diseases, and other pathological conditions.
Antibodies targeting RNF7 are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly used in techniques such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to detect endogenous RNF7 in various cell types and tissues. High-quality RNF7 antibodies are validated for specificity, often through knockout or knockdown controls, to ensure accurate detection of the ~14 kDa protein. Researchers utilize these antibodies to explore RNF7's role in ubiquitination pathways, its association with Cul5-dependent substrate recognition, and its potential as a therapeutic target in diseases linked to protein homeostasis imbalance. Commercial RNF7 antibodies are typically raised in hosts like rabbits or mice, with reactivity across human, mouse, and rat samples.
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