| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ASE1; PRC1; Protein regulating cytokinesis 1;;PRC1 |
| WB Predicted band size | 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PRC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PRC1抗体的代表性文献(模拟示例，非真实文献，供参考)：
1. **文献名称**：*PRC1 maintains transcriptional repression of Hox genes through DNA methylation*
**作者**：Cao R, et al.
**摘要**：该研究利用PRC1特异性抗体进行ChIP-seq分析，发现PRC1通过调控DNA甲基化维持Hox基因沉默，揭示了其在胚胎发育中的表观遗传调控机制。
2. **文献名称**：*A monoclonal antibody against PRC1 highlights its overexpression in hepatocellular carcinoma*
**作者**：Peng J, et al.
**摘要**：通过开发PRC1单克隆抗体，研究发现PRC1在肝癌组织中高表达，并与患者预后不良相关，提示其作为癌症生物标志物的潜力。
3. **文献名称**：*PRC1 interacts with Cyclin B1 and regulates mitotic progression*
**作者**：Ismail IH, et al.
**摘要**：使用PRC1抗体进行免疫共沉淀实验，证实PRC1与Cyclin B1相互作用，调控细胞有丝分裂进程，为癌症靶向治疗提供新靶点。
*注：以上内容为模拟示例，实际文献需通过PubMed/Google Scholar检索确认。*
The PRC1 (Polycomb Repressive Complex 1) antibody is a key tool for studying epigenetic regulation mediated by the Polycomb group (PcG) proteins, which are essential for maintaining transcriptional repression of developmental genes. PRC1. part of the larger Polycomb repressive system, works in concert with PRC2 (Polycomb Repressive Complex 2) to establish and stabilize chromatin compaction. While PRC2 catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), PRC1 recognizes this mark and facilitates monoubiquitination of histone H2A at lysine 119 (H2AK119ub), reinforcing chromatin silencing. This interplay ensures stable gene repression during cellular differentiation, stem cell maintenance, and development.
PRC1 antibodies are widely used to investigate its localization, expression levels, and functional roles in diverse biological contexts, including cancer, where dysregulated PRC1 activity is linked to aberrant cell proliferation and tumorigenesis. These antibodies enable techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting, aiding in mapping PRC1’s genome-wide interactions and its association with disease states. Research utilizing PRC1 antibodies has also explored its non-canonical roles in DNA repair and cell cycle regulation. As PRC1-targeted therapies emerge, these antibodies are critical for validating drug efficacy and understanding resistance mechanisms. Their application spans basic epigenetics, developmental biology, and translational oncology, underscoring their importance in both mechanistic and clinical studies.
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