| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20; ;RNF20 |
| WB Predicted band size | 114 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human RNF20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于RNF20抗体的代表性文献(内容基于真实研究，但文献信息为模拟概括)：
1. **"RNF20 mediates histone H2B ubiquitination and regulates transcriptional elongation"**
*Authors: Kim J, et al.*
摘要：该研究利用RNF20抗体，通过ChIP实验证明RNF20催化组蛋白H2B的K120位点泛素化，调控RNA聚合酶II介导的转录延伸，影响基因表达全局水平。
2. **"RNF20 suppresses tumor progression and metastatic adaptation via epigenetic regulation of autophagy"**
*Authors: Shema E, et al.*
摘要：研究通过Western blot和免疫组化(使用RNF20抗体)发现，RNF20作为肿瘤抑制因子，通过抑制自噬相关基因的表观沉默，阻碍乳腺癌细胞的转移和耐药性。
3. **"RNF20 recruits USP44 to deubiquitinate H2B and maintain genome stability"**
*Authors: Zhang F, et al.*
摘要：该文献使用RNF20抗体进行共免疫沉淀实验，揭示RNF20与去泛素化酶USP44形成复合物，动态调控H2B泛素化水平，从而维持DNA损伤修复和基因组稳定性。
注：以上文献标题和摘要根据领域内典型研究方向模拟，实际引用时建议通过PubMed或Google Scholar以“RNF20 antibody”为关键词检索最新实证研究。
RNF20. also known as BRE1A, is an E3 ubiquitin ligase that plays a critical role in histone H2B monoubiquitination (H2Bub1), a post-translational modification essential for chromatin remodeling and transcriptional regulation. As a member of the RING finger protein family, RNF20 forms a heterodimer with RNF40 to mediate the ubiquitination of histone H2B at lysine 120. a process vital for DNA damage repair, cell cycle progression, and gene expression. Dysregulation of RNF20 has been implicated in cancer, where its loss or reduced expression correlates with tumor progression, metastasis, and poor prognosis.
RNF20 antibodies are essential tools for studying its expression, localization, and molecular functions. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate RNF20's role in epigenetic regulation, oncogenesis, and DNA repair mechanisms. High-quality RNF20 antibodies exhibit specificity for targeting distinct epitopes, enabling researchers to distinguish RNF20 from related proteins. Validation via knockout cell lines or siRNA-mediated knockdown is critical to confirm antibody reliability.
Research utilizing RNF20 antibodies has advanced understanding of its tumor-suppressive activities, including modulation of oncogene _expression (e.g., c-Fos, c-Jun) and cooperation with p53 in stress responses. These studies highlight RNF20's potential as a therapeutic target or biomarker in cancer and other diseases linked to epigenetic dysregulation.
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