| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin; LRP2; DBS;;LRP2 |
| WB Predicted band size | Calculated MW: 522 kDa ; Observed MW: 280 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LRP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于 **Lrp2/Megalin抗体** 的参考文献摘要(精简版)：
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1. **文献名称**: *"Megalin and Cubilin in proximal tubule protein reabsorption: from experimental models to human disease"*
**作者**: Nielsen R, Christensen EI, Birn H.
**摘要**: 本文综述了Megalin(Lrp2)与Cubilin在肾近端小管内吞途径中的协同作用，重点探讨其在蛋白质重吸收中的功能，并分析了其基因突变或表达异常与慢性肾脏病中蛋白尿的相关机制。
2. **文献名称**: *"Lrp2/Megalin is required for the early steps of retinal development"*
**作者**: Dietzel E, et al.
**摘要**: 研究通过基因敲除模型发现，Megalin在中枢神经系统发育中(尤其是视网膜形成阶段)对信号分子(如SHH、WNT)的转运至关重要，其缺失会导致神经上皮发育异常。
3. **文献名称**: *"Megalin-dependent clearance of amyloid beta by the kidney"*
**作者**: Yamamoto SY, et al.
**摘要**: 该研究揭示了Megalin在肾脏清除血液中β-淀粉样蛋白(Aβ)的关键作用，利用Megalin抗体阻断实验证实其内吞功能缺陷可能加重Aβ沉积，与阿尔茨海默病和肾脏病变相关。
4. **文献名称**: *"Megalin-mediated drug uptake in renal proximal tubules: implications for nephrotoxicity"*
**作者**: Moestrup SK, Verkman AS.
**摘要**: 研究利用特异性Megalin抗体，证明了该受体在介导肾小管对氨基糖苷类抗生素等药物的重吸收中起核心作用，其过度激活可能导致药物蓄积性肾损伤。
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以上文献涵盖了Megalin在生理功能、发育、疾病机制及药物代谢中的研究，均涉及抗体实验的应用。如需具体期刊年份或DOI，可进一步补充检索条件。
**Background of Lrp2/Megalin Antibodies**
Lrp2 (low-density lipoprotein receptor-related protein 2), also known as Megalin, is a large transmembrane glycoprotein belonging to the LDL receptor family. It is primarily expressed in polarized epithelial cells, such as renal proximal tubules, alveolar epithelia, and the inner ear. Megalin functions as an endocytic receptor, facilitating the uptake of diverse ligands, including vitamins, hormones, lipoproteins, and signaling molecules, playing critical roles in nutrient absorption, cellular signaling, and tissue homeostasis.
Antibodies targeting Megalin are essential tools for studying its expression, localization, and function in physiological and pathological contexts. In research, these antibodies are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate Megalin's role in diseases such as chronic kidney disease (e.g., diabetic nephropathy), hearing loss, and neurodegenerative disorders like Alzheimer’s disease, where its interaction with amyloid-β is implicated. Additionally, Megalin's involvement in drug transport (e.g., aminoglycoside antibiotics) and vitamin metabolism (e.g., vitamin D) makes its antibodies valuable in pharmacokinetic and metabolic studies.
Commercial Megalin antibodies are often raised against specific epitopes, such as extracellular or cytoplasmic domains, with validation in knockout models to ensure specificity. Their application spans renal physiology, neurobiology, and cancer research, particularly in cancers overexpressing Megalin. Understanding Megalin's multifaceted roles through antibody-based assays continues to advance therapeutic targeting and biomarker discovery.
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