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产品描述：人吲哚胺2,3-双加氧酶(IDO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IDO1含量。IDO1即吲哚胺2,3 -双加氧酶1，是肿瘤免疫治疗中的热门靶点。它可通过催化色氨酸沿犬尿氨酸途径代谢，造成肿瘤微环境中色氨酸耗竭，抑制T细胞活性和功能，从而帮助肿瘤细胞免疫逃逸，目前针对它的研究聚焦于开发抑制剂以增强抗肿瘤免疫。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于体外研究 IDO 在免疫逃逸机制中的作用、药物干预对 IDO 活性的影响，或评估炎症性疾病及癌症模型中 IDO 的表达水平，为免疫调节相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：3-dioxygenase ELISA Kit; I23O1_HUMAN ELISA Kit; IDO 1 ELISA Kit; IDO ELISA Kit; IDO-1 ELISA Kit; IDO1 ELISA Kit; INDO ELISA Kit; indolamine 2,3 dioxygenase ELISA Kit; Indole 2 3 dioxygenase ELISA Kit; indoleamine 2 3 dioxygenase 1 ELISA Kit; indoleamine 2 3 dioxygenase ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; Indoleamine pyrrole 2 3 dioxygenase ELISA Kit; Indoleamine-pyrrole 2 ELISA Kit
缩写：IDO1
Uniprot No.：P14902
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-104 | ||||
| EDTA plasma (n=4) | 86 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.5742.5522.5632.461252.0781.9492.0141.91212.51.2281.2781.2531.1516.250.5370.5600.5490.4473.120.3260.3400.3330.2311.560.2040.1970.2010.0990.780.1590.1520.1560.05400.1030.1000.102 | ||||||
货期：3-5 working days
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