| WB | 咨询技术 | Recombinant protein |
| IF | 咨询技术 | Recombinant protein |
| IHC | 咨询技术 | Recombinant protein |
| ICC | 1/100-1/500 | Recombinant protein |
| FCM | 咨询技术 | Recombinant protein |
| Elisa | 咨询技术 | Recombinant protein |
| Aliases | GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611; RFP;;RFP tag |
| WB Predicted band size | Calculated MW: 26 kDa ; Observed MW: Recombinant protein dependent |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Recombinant protein |
| Immunogen | Recombinant Protein of GFP RFP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与RFP抗体相关的参考文献及其摘要概括：
1. **"A monoclonal antibody for red fluorescent protein enables immunolabeling in light and electron microscopy"**
*作者：C. J. Pedelacq 等 (2006)*
*摘要：* 该研究报道了一种特异性识别RFP的单克隆抗体的开发，验证了其在荧光显微镜和免疫电镜中的应用，证实其可用于检测RFP标记的蛋白质在细胞内的定位。
2. **"Versatile protein tagging in cells with split fluorescent protein"**
*作者：T. Kamiyama 等 (2016)*
*摘要：* 文章提出一种基于拆分RFP的蛋白质标记系统，并利用RFP抗体验证了标记效率。该技术可用于实时追踪蛋白质相互作用，抗体在Western blot和免疫沉淀中表现出高特异性。
3. **"Immunohistochemical detection of fluorescent reporter proteins in tissue sections"**
*作者：B. D. Riedel 等 (2011)*
*摘要：* 研究优化了针对RFP的免疫组化方法，比较了不同抗体的灵敏度，证明特定RFP抗体在石蜡包埋组织中仍能有效检测低丰度的RFP融合蛋白，适用于病理学研究。
4. **"High-efficiency transfection and drug selection of genetically modified cells using red fluorescent protein"**
*作者：M. Yang 等 (2000)*
*摘要：* 利用RFP抗体筛选高表达RFP的转基因细胞系，验证了抗体在流式细胞分选和蛋白质印迹中的可靠性，为基因治疗研究提供了工具支持。
(注：以上文献标题与作者为虚构示例，仅用于展示回答格式，实际文献需通过学术数据库检索。)
Red fluorescent protein (RFP) antibodies are immunological tools designed to detect and localize RFP-tagged proteins in experimental systems. RFP, originally derived from the coral *Discosoma* sp., is a β-barrel-structured fluorescent protein that emits red light (λ~558-583 nm) when excited. Its development expanded the color palette of fluorescent markers beyond green fluorescent protein (GFP), enabling multicolor imaging and improved tissue penetration in microscopy.
RFP antibodies are typically generated by immunizing hosts (e.g., rabbits, mice) with recombinant RFP or its variants (e.g., mCherry, tdTomato). These antibodies exhibit high specificity for RFP epitopes, allowing applications in Western blotting, immunofluorescence, flow cytometry, and immunohistochemistry. They are particularly valuable in tracking RFP-fusion proteins to study subcellular localization, protein-protein interactions, or gene expression dynamics in live or fixed cells.
The utility of RFP antibodies extends to validating CRISPR/Cas9-edited cell lines (confirming RFP knock-in/knockout) and analyzing transgenic organisms engineered with RFP reporters. Commercial RFP antibodies are often validated for cross-reactivity with common RFP derivatives, though batch-specific verification remains critical. As red fluorescence minimizes autofluorescence interference in biological samples, RFP-based systems paired with specific antibodies continue to advance research in cell biology, neuroscience, and developmental studies, offering spatial and temporal resolution in complex experimental models.
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