| WB | 咨询技术 | Yeast(Saccharomycescerevisiae) |
| IF | 咨询技术 | Yeast(Saccharomycescerevisiae) |
| IHC | 咨询技术 | Yeast(Saccharomycescerevisiae) |
| ICC | 技术咨询 | Yeast(Saccharomycescerevisiae) |
| FCM | 咨询技术 | Yeast(Saccharomycescerevisiae) |
| Elisa | 咨询技术 | Yeast(Saccharomycescerevisiae) |
| Aliases | Alpha-tubulin 1;H2 ALPHA;LIS3;TUBA1;TUBA1A;Tuba4a;Tubulin alpha-4A chain;alpha Tubulin |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Yeast(Saccharomycescerevisiae) |
| Immunogen | A synthesized peptide derived from alpha Tubulin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于酵母alpha-Tubulin抗体的参考文献摘要(虚构示例，仅供参考)：
1. **"Yeast Tubulin Genes: Isolation and Characterization"**
*Huffaker, T.C., et al. (1987)*
该研究克隆了酵母α-tubulin编码基因TUB1.并制备了多克隆抗体用于定位α-tubulin在酵母细胞周期中的动态分布，验证了抗体在免疫荧光和Western blot中的特异性。
2. **"Assembly of Microtubules in Fission Yeast"**
*Drubin, D.G. & Kirschner, M.W. (1986)*
通过纯化裂殖酵母α-tubulin蛋白并生成单克隆抗体，揭示了微管体外组装机制，抗体被用于体外结合实验及活细胞微管结构成像。
3. **"Spindle Pole Body Dynamics in Budding Yeast"**
*Byers, B. & Goetsch, L. (1975)*
早期利用α-tubulin抗体的经典研究，通过免疫电镜技术解析了酵母纺锤体极体(SPB)与微管骨架的相互作用，为酵母有丝分裂研究奠定基础。
4. **"Methods in Yeast Cell Biology"**
*Pringle, J.R., et al. (1991)*
综述中详细描述了α-tubulin抗体在酵母细胞裂解物标准化中的应用(如Western blot内参)，并提供了抗体使用条件和交叉反应验证数据。
注：以上为模拟示例，实际文献需通过PubMed或Google Scholar以关键词“alpha-tubulin antibody yeast”检索获取。
AlphaTubulin is a highly conserved structural protein essential for forming microtubules, dynamic cytoskeletal components critical for various cellular processes including chromosome segregation during mitosis, intracellular transport, and maintenance of cell shape. In yeast, alpha-tubulin (encoded by the TUB1 and TUB3 genes in budding yeast, or the atb2+ gene in fission yeast) pairs with beta-tubulin to form heterodimers, the building blocks of microtubules. Due to its conserved nature and functional significance, alpha-tubulin serves as a widely used loading control in Western blotting and a marker for microtubule visualization in immunofluorescence studies.
Antibodies targeting yeast alpha-tubulin are specifically developed to recognize epitopes unique to fungal tubulin isoforms, minimizing cross-reactivity with mammalian homologs. These antibodies are crucial tools in studying yeast cell biology, particularly in experiments exploring cell cycle regulation, microtubule dynamics, or responses to anti-microtubule drugs (e.g., benomyl). They enable researchers to monitor tubulin expression levels, post-translational modifications (e.g., acetylation, tyrosination), and microtubule organization under varying genetic or environmental conditions. Both monoclonal and polyclonal versions are available, often validated for applications like immunoblotting, immunoprecipitation, and fluorescence microscopy in Saccharomyces cerevisiae or Schizosaccharomyces pombe models. Their reliability in quantitative assays makes them indispensable for normalizing protein samples and ensuring experimental reproducibility in yeast studies.
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