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产品描述：兔子细胞间粘附分子1(ICAM-1/CD54)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ICAM-1/CD54含量。ICAM-1/CD54是重要细胞黏附分子。其背景为在多种细胞表面表达，参与免疫应答、炎症反应等生理病理过程。研究机制上，它能介导细胞间黏附与信号传导，异常表达与多种疾病如炎症性疾病、肿瘤转移等密切相关，是潜在治疗靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，兔类动物模型中炎症相关疾病机制研究、免疫调节药物开发评估、感染病理进程分析、心血管疾病模型、自身免疫性疾病研究、病毒/细菌感染实验中的生物标志物动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ICAM-1/CD54
Uniprot No.：U3KPG6
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:10 | Average % | 99 | ||||
| Range % | 91-106 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 82-94 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 100 | 96-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6942.5942.6442.501202.1442.0442.0941.951101.3241.2841.3041.16150.7540.7340.7440.6012.50.5360.5460.5410.3981.250.3510.3610.3560.2130.6250.2200.2300.2250.08200.1420.1430.143 | ||||||
货期：3-5 working days
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