首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠羟脯氨酸(Hyp)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Hyp含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，在纤维化疾病机制研究、组织修复评估及骨代谢调控等领域具有重要科研价值；可高效检测大鼠模型（如肝纤维化、肺纤维化）中胶原沉积程度；用于皮肤创伤修复、骨关节炎等研究中的组织代谢动态监测；为探索Hyp相关分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Hyp
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:1600 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of rat Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 100.1120.1150.114 50.1860.1790.183 2.50.2600.2690.265 1.250.4710.4390.455 0.6250.6150.6290.622 0.3121.1171.1391.128 0.1562.1732.2292.201 02.8542.8972.876 | ||||||
货期：3-5 working days
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