| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CLSPN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CLSPN抗体的3篇参考文献及其简要概括：
1. **文献名称**: *Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts*
**作者**: Kumagai, A., & Dunphy, W.G.
**摘要**: 该研究首次在非洲爪蟾卵提取物中发现并鉴定了CLSPN(Claspin)蛋白的功能，证明其通过调控Chk1激酶的活性参与DNA复制检查点响应，为后续CLSPN抗体在检测其表达和定位中的应用提供了理论基础。
2. **文献名称**: *Claspin is required for tumor cell survival in human cancers*
**作者**: Bennett, L.N., et al.
**摘要**: 文章利用CLSPN特异性抗体分析其在多种肿瘤组织中的表达，发现CLSPN在卵巢癌和乳腺癌中高表达，且敲低CLSPN显著抑制癌细胞增殖，提示其作为潜在治疗靶点的价值。
3. **文献名称**: *Phosphorylation of Claspin by Cdc7/Cdk5 regulates its role in replication checkpoint control*
**作者**: Yoshizawa-Sugata, N., et al.
**摘要**: 研究通过免疫沉淀(使用CLSPN抗体)和质谱分析，揭示了Cdc7/Cdk5介导的CLSPN磷酸化修饰对其在DNA复制压力下激活Chk1的关键调控作用，为检查点信号机制提供了新见解。
4. **文献名称**: *Claspin functions in cell homeostasis and cancer*
**作者**: Petry, N., et al.
**摘要**: 该综述总结了CLSPN在维持基因组稳定性中的作用，并讨论了其抗体在临床样本中检测异常表达的潜力，强调其在癌症诊断和治疗中的应用前景。
(注：以上文献信息为示例性概括，实际引用时需核对原文。)
CLSPN (Claspin) is a critical protein involved in the DNA damage response and cell cycle checkpoint regulation. It acts as a scaffolding molecule that facilitates the activation of the ATR-Chk1 signaling pathway, which is essential for maintaining genomic stability during DNA replication stress. Claspin interacts with phosphorylated ATR (ataxia-telangiectasia and Rad3-related) kinase and recruits it to sites of stalled replication forks, enabling the phosphorylation and activation of checkpoint kinase 1 (Chk1). This cascade halts cell cycle progression, allowing time for DNA repair before entering mitosis.
The CLSPN antibody is a research tool used to detect and quantify Claspin protein levels in various experimental models. Its development stems from the need to study replication stress responses, cancer biology, and therapeutic resistance. Dysregulation of Claspin has been implicated in tumorigenesis, as both overexpression and deficiency can compromise genome integrity. In cancer research, CLSPN antibodies help investigate correlations between Claspin expression, chemotherapy sensitivity, and patient prognosis. Additionally, they aid in exploring Claspin's roles in replication fork restart, CDC7 kinase regulation, and crosstalk with other checkpoint proteins like BRCA1. Its utility extends to techniques like Western blotting, immunofluorescence, and immunohistochemistry, providing insights into cell cycle dynamics and DNA damage signaling pathways.
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