| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TOP2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于TOP2B抗体的参考文献示例(内容为虚构，仅作格式参考)：
1. **"TOP2B in Neural Development: Antibody Validation and Functional Insights"**
- Authors: Smith A, et al.
- 摘要：研究验证了TOP2B抗体在神经元分化模型中的特异性，发现TOP2B通过调控染色质结构影响神经突触形成。
2. **"TOP2B as a Biomarker in Triple-Negative Breast Cancer: Immunohistochemical Analysis"**
- Authors: Lee C, et al.
- 摘要：通过TOP2B抗体检测乳腺癌组织，证实其高表达与化疗耐药性相关，提示其作为预后标志物的潜力。
3. **"TOP2B-Mediated DNA Damage Repair in Alzheimer’s Disease Models"**
- Authors: Garcia R, et al.
- 摘要：利用TOP2B抗体揭示其在阿尔茨海默病神经元中异常激活，导致tau蛋白病理积累的分子机制。
4. **"Comparative Study of Commercial TOP2B Antibodies for Chromatin Immunoprecipitation"**
- Authors: Patel K, et al.
- 摘要：评估多种商业TOP2B抗体的ChIP效率，为表观遗传学研究提供抗体选择指南。
(注：以上文献为示例，实际引用需查询PubMed等数据库获取真实信息。)
TOP2B (DNA topoisomerase II beta) is a nuclear enzyme belonging to the type II topoisomerase family, which plays a critical role in modulating DNA topology during replication, transcription, and chromosome segregation. It introduces transient double-strand breaks in DNA, enabling the resolution of torsional stress and maintaining genomic stability. Unlike its isoform TOP2A, which is cell cycle-dependent and highly expressed in proliferating cells, TOP2B is ubiquitously expressed in both dividing and post-mitotic cells, particularly in neurons, where it supports transcriptional regulation and neural development.
Antibodies targeting TOP2B are essential tools in biomedical research, enabling the investigation of its cellular functions and dysregulation in diseases. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study TOP2B expression patterns, localization, and interactions. In cancer research, TOP2B antibodies help explore its dual role as a potential oncogene or tumor suppressor, depending on cellular context. Aberrant TOP2B activity has been linked to chemoresistance and neurodegenerative disorders, such as Alzheimer’s disease, due to its involvement in DNA repair and neuronal survival pathways. Additionally, these antibodies aid in evaluating the efficacy of topoisomerase-targeting drugs, including etoposide, which indirectly affects TOP2B. Despite challenges in isoform specificity due to structural similarities with TOP2A, optimized TOP2B antibodies remain crucial for deciphering its biological and clinical significance.
×
