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产品描述：大鼠血清素受体2A/5-羟色胺受体2A(5-HT-2A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HTR2A含量。HTR2A，即5-羟色胺2A受体，是一种G蛋白偶联受体，广泛分布于大脑，与情绪、认知等功能密切相关。其基因多态性与多种疾病如抑郁症、精神分裂症等密切相关，是精神药理学研究和相关疾病治疗的重要靶点。研究机制主要涉及HTR2A与5-羟色胺结合，激活G蛋白，进而调节下游效应子的活性。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经药理学研究、动物行为学模型评估、神经递质受体动态分析及精神疾病机制探索等科研场景。为研究5-HT-2A受体在神经信号传导、药物干预响应中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Htr2a; Htr2; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; Serotonin receptor 2A
缩写：HTR2A
Uniprot No.：P14842
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT-2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-110 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat 5-HT-2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-96 | ||||
| EDTA plasma (n=4) | 95 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1212.0952.1082.00410001.8741.8581.8661.7625001.5461.5331.5401.4362501.1201.1021.1111.0071250.7070.7180.7130.60962.50.4980.5160.5070.40331.250.2950.2800.2880.18400.1040.1030.104 | ||||||
货期：3-5 working days
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