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Mouse Monoclonal POLR1G Antibody

  • 中文名： POLR1G抗体
  • 别    名： nan
货号: IPDX16203
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human POLR1G
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是模拟的关于POLR1G抗体的参考文献示例(实际文献需通过学术数据库检索确认)：

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1. **文献名称**：*POLR1G Antibody Validation and Its Role in Ribosome Biogenesis*

**作者**：Zhang Y, et al.

**摘要**：本研究开发并验证了一种高特异性兔源多克隆POLR1G抗体，通过Western blot和免疫荧光实验证实其可识别内源性POLR1G蛋白，定位在细胞核仁中。抗体进一步用于探究POLR1G在核糖体RNA前体加工中的功能。

2. **文献名称**：*Overexpression of POLR1G Predicts Poor Prognosis in Hepatocellular Carcinoma*

**作者**：Wang L, et al.

**摘要**：通过免疫组化(使用抗POLR1G抗体)分析肝癌组织样本，发现POLR1G蛋白表达水平升高与肿瘤分期和患者生存率下降显著相关，提示其作为潜在预后标志物的可能性。

3. **文献名称**：*Interaction Mapping of RNA Polymerase I Subunits via Co-Immunoprecipitation*

**作者**：Johnson RB, et al.

**摘要**：利用POLR1G抗体进行免疫共沉淀实验，鉴定了POLR1G与其他RNA聚合酶I亚基(如POLR1A和POLR1B)的相互作用网络，揭示了其在复合体组装中的关键作用。

4. **文献名称**：*POLR1G Regulates Embryonic Stem Cell Differentiation by Modulating rRNA Synthesis*

**作者**：Kim S, et al.

**摘要**：通过siRNA敲低和POLR1G抗体介导的蛋白检测，证明POLR1G通过调控rRNA合成影响胚胎干细胞的自我更新和分化能力。

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**提示**：以上为示例，实际文献建议通过PubMed、Google Scholar等平台以关键词“POLR1G antibody”或“RNA polymerase I subunit G”检索，并筛选涉及抗体开发、验证或应用的研究。

背景信息

The POLR1G antibody targets the protein encoded by the POLR1G gene, a key component of RNA polymerase I (Pol I), which is essential for ribosomal RNA (rRNA) synthesis. POLR1G, also known as RPA34. is a core subunit of Pol I, forming part of the enzyme’s catalytic core alongside POLR1D (RPA12). This subunit plays a critical role in Pol I assembly, stability, and transcriptional activity, particularly during rRNA precursor synthesis in the nucleolus. Dysregulation of POLR1G has been linked to developmental disorders and diseases, including Treacher Collins syndrome (a craniofacial disorder) and 3C syndrome (cerebellar hypoplasia, cataracts, and cognitive impairment), often caused by mutations in POLR1G or related Pol I subunits. Additionally, POLR1G overexpression has been observed in certain cancers, suggesting potential roles in tumorigenesis. Antibodies against POLR1G are widely used in research to study Pol I structure, rRNA transcription mechanisms, and disease pathogenesis. They enable detection of POLR1G expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in understanding its cellular localization and interactions. Such tools are crucial for exploring POLR1G’s functional contributions to ribosome biogenesis and its implications in congenital disorders and cancer, providing insights for therapeutic targeting.

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