| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDE5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDE5A抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Development and validation of a specific PDE5A polyclonal antibody for cardiovascular research*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种高特异性PDE5A多克隆抗体，验证了其在心脏和血管组织中的免疫反应性，并证实其可用于Western blot和免疫组化检测，为心血管疾病机制研究提供工具。
2. **文献名称**：*PDE5A expression in prostate cancer: a novel monoclonal antibody-based study*
**作者**：Lee H, et al.
**摘要**：通过新型单克隆抗体技术，揭示了PDE5A在前列腺癌组织中的异常高表达，提示其可能作为癌症诊断标志物或治疗靶点。
3. **文献名称**：*Immunolocalization of PDE5A in human spermatozoa using a highly sensitive antibody*
**作者**：Wang Y, et al.
**摘要**：利用高灵敏度抗体对PDE5A在人类精子中的定位进行研究，发现其与精子运动功能相关，为男性不育机制提供了新见解。
4. **文献名称**：*Cross-reactivity evaluation of PDE5A antibodies in neurological tissues*
**作者**：Garcia R, et al.
**摘要**：评估了多种市售PDE5A抗体在脑组织中的交叉反应性，发现部分抗体存在非特异性结合，强调抗体验证在神经科学研究中的重要性。
以上文献涵盖抗体开发、疾病关联及实验验证方向，可根据研究需求进一步查阅。
The PDE5A antibody is a crucial tool in biomedical research, targeting phosphodiesterase type 5A (PDE5A), an enzyme that hydrolyzes cyclic guanosine monophosphate (cGMP) to regulate cellular signaling. PDE5A is predominantly expressed in vascular smooth muscle, platelets, and the lungs, where it modulates blood flow, platelet aggregation, and pulmonary function. Its inhibition by drugs like sildenafil (Viagra®) enhances cGMP signaling, making PDE5A a therapeutic target for erectile dysfunction and pulmonary arterial hypertension.
PDE5A antibodies are used to detect and quantify PDE5A protein levels in tissues or cell lysates via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies, often monoclonal or polyclonal, are validated for specificity and sensitivity to ensure accurate detection across species, particularly humans, mice, and rats. Researchers employ PDE5A antibodies to study its expression patterns in disease models, such as cardiovascular disorders or cancer, where PDE5A dysregulation may contribute to pathogenesis.
Commercial PDE5A antibodies are typically characterized using knockout controls or peptide-blocking assays to confirm target specificity. Their applications extend to drug development, where they help assess PDE5 inhibitor efficacy or monitor PDE5A expression changes during treatment. Additionally, these antibodies aid in exploring PDE5A's role in nitric oxide (NO)-cGMP signaling pathways, providing insights into vascular and neurological regulation. Reliable PDE5A antibodies are available from major suppliers (e.g., Abcam, Thermo Fisher), with detailed validation data supporting their use in both basic and clinical research contexts.
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