| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human KHK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KHK(酮己糖激酶)抗体的3篇模拟参考文献(内容为假设性描述，实际文献需通过学术数据库核实)：
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1. **文献名称**：*Development of a Monoclonal Antibody Specific for Human Ketohexokinase and Its Application in Metabolic Disease Models*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种高特异性单克隆抗体，用于检测人源KHK蛋白在肝脏和肾脏组织中的表达。通过免疫印迹和免疫组化验证，该抗体成功应用于高果糖饮食诱导的小鼠代谢综合征模型，揭示了KHK在果糖代谢异常中的关键作用。
2. **文献名称**：*Differential Expression of KHK Isoforms in Diabetes: Insights from a Novel Polyclonal Antibody Approach*
**作者**：Chen L, et al.
**摘要**：作者制备了针对KHK两种亚型(KHK-A和KHK-C)的多克隆抗体，并发现2型糖尿病患者脂肪组织中KHK-C表达显著上调。该抗体为研究KHK亚型在胰岛素抵抗中的功能差异提供了重要工具。
3. **文献名称**：*KHK Antibody-Based Detection of Fructokinase Activity in Cardiovascular Tissues*
**作者**：Rodriguez M, et al.
**摘要**：利用新型KHK抗体结合酶活性分析，研究揭示了血管内皮细胞中果糖激酶的异常活化与动脉粥样硬化进展的相关性，为心血管疾病中果糖代谢的靶向治疗提供了依据。
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**注意**：以上内容为示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“KHK antibody”“fructokinase antibody”)获取。如需具体文献，请提供更详细的研究方向或应用场景。
KHK (ketohexokinase) antibodies are primarily associated with research focused on the enzyme ketohexokinase, which plays a critical role in fructose metabolism. KHK, also known as fructokinase, catalyzes the phosphorylation of fructose to fructose-1-phosphate, a key step in its metabolic pathway. Dysregulation of fructose metabolism has been linked to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD), obesity, and diabetes, making KHK a subject of interest in biomedical research.
KHK exists in two isoforms, KHK-A and KHK-C, generated by alternative splicing. KHK-C, the predominant isoform in the liver, kidneys, and small intestine, exhibits higher enzymatic activity, while KHK-A is expressed in other tissues with lower catalytic efficiency. Antibodies targeting KHK are essential tools for studying its expression, localization, and function in both normal physiology and disease contexts. They are used in techniques like Western blotting, immunohistochemistry, and ELISA to quantify protein levels or assess tissue-specific distribution.
Recent studies have explored KHK inhibition as a therapeutic strategy for metabolic diseases, driving demand for reliable antibodies to evaluate drug efficacy and mechanistic outcomes. Additionally, KHK antibodies aid in investigating genetic variants or mutations affecting enzyme activity. Their application extends to preclinical models, helping unravel the role of fructose metabolism in inflammation, insulin resistance, and cellular stress. As fructose consumption rises globally, understanding KHK's pathophysiology remains crucial, positioning these antibodies as vital reagents in metabolic research.
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