首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠热休克蛋白90-alpha(Hsp90aa1/Hsp86/Hspca)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Hsp90aa1含量。Hsp90aa1 是一种重要靶点。它在细胞应激反应、蛋白折叠等方面发挥关键作用。研究聚焦其与肿瘤发生发展关联，通过抑制它的活性，干扰肿瘤细胞的信号通路、影响蛋白稳定性，为开发新型抗癌药物提供了理论基础和潜在方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研实验中评估大鼠模型在热应激、氧化损伤或疾病状态下Hsp90aa1的动态变化，为探究其分子调控机制、筛选潜在调控药物提供可靠工具；支持研究者高效开展心血管疾病、代谢综合征等基础医学领域的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit
缩写：HSP-90
Uniprot No.：P82995
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hsp90aa1/Hsp86/Hspca in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-95 | |||||
回收率：
| The recovery of rat Hsp90aa1/Hsp86/Hspca spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 101 | 97-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5172.7352.6262.433101.7721.8591.8161.62351.1591.1641.1620.9692.50.6800.7400.7100.5171.250.5170.5390.5280.3350.6250.3740.3880.3810.1880.3120.2900.3140.3020.10900.1890.1970.193 | ||||||
货期：3-5 working days
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