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产品描述：人热休克蛋白糖蛋白96(HSP gp96)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP gp96含量。HSP gp96，即热休克蛋白gp96，是一种高度保守的分子伴侣，参与蛋白质折叠、转运和合成。研究发现其在多种肿瘤中表达升高，与肿瘤的发生发展密切相关。HSP gp96通过参与MHC I类抗原的提呈，激活CD8+细胞毒性T细胞，从而在抗肿瘤免疫中发挥重要作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，肿瘤免疫治疗机制探索、应激相关疾病模型建立、免疫细胞功能研究及药物靶点筛选等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：94 kDa glucose regulated protein ELISA Kit; 94 kDa glucose-regulated protein ELISA Kit; ECGP ELISA Kit; Endoplasmin ELISA Kit; Endothelial cell (HBMEC) glycoprotein ELISA Kit; ENPL_HUMAN ELISA Kit; Glucose regulated protein 94kDa ELISA Kit; gp96 ELISA Kit; gp96 homolog ELISA Kit; GRP 94 ELISA Kit; GRP-94 ELISA Kit; Heat shock protein 90 kDa beta member 1 ELISA Kit; heat shock protein 90kDa beta (Grp94); member 1 ELISA Kit; Heat shock protein; 90 kDa; beta; 1 ELISA Kit; HSP90B1 ELISA Kit; Stress inducible tumor rejection antigen GP96 ELISA Kit; TRA1 ELISA Kit; tumor rejection antigen (gp96) 1 ELISA Kit; Tumor rejection antigen 1 ELISA Kit; Tumor rejection antigen gp96 ELISA Kit; Tumor rejection antigen-1 (gp96) ELISA Kit
缩写：HSP gp96
Uniprot No.：P14625
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP gp96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-108 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human HSP gp96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.1722.2492.2112.089 201.6721.7301.7011.579 101.1011.1041.1030.981 50.6260.5930.6100.488 2.50.4090.4040.4070.285 1.250.3020.2890.2960.174 0.6250.2250.2190.2220.100 00.1270.1170.122 | ||||||
货期：3-5 working days
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