WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/250 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human STAG3 |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于STAG3抗体的3篇虚构参考文献示例(内容为模拟,非真实文献):
1. **文献名称**:*STAG3 regulates meiotic cohesion in mammalian germ cells*
**作者**:Smith A, et al.
**摘要**:研究利用STAG3特异性抗体,揭示了STAG3蛋白在小鼠减数分裂中维持染色体黏连的关键作用,缺失导致配子形成异常和不育。
2. **文献名称**:*A novel STAG3 antibody reveals impaired cohesin complex in human infertility*
**作者**:Chen L, et al.
**摘要**:开发了一种高特异性STAG3单克隆抗体,用于检测人类睾丸组织中黏连蛋白复合体完整性,发现STAG3表达缺失与男性无精症显著相关。
3. **文献名称**:*CRISPR screening identifies STAG3 as essential for oocyte development*
**作者**:Wang Y, et al.
**摘要**:通过CRISPR筛选结合STAG3抗体免疫荧光验证,证明STAG3缺失破坏卵母细胞减数分裂纺锤体组装,导致胚胎发育停滞。
4. **文献名称**:*Structural analysis of STAG3 cohesin subunit using epitope-specific antibodies*
**作者**:Garcia R, et al.
**摘要**:利用表位特异性STAG3抗体解析其与黏连蛋白其他亚基的互作界面,为染色体分离异常的分子机制提供结构依据。
(注:以上文献为示例性质,实际研究中请通过PubMed等数据库查询真实发表论文。)
The STAG3 antibody targets the STAG3 protein, a subunit of the cohesin complex, which plays critical roles in chromosome segregation, DNA repair, and transcriptional regulation during cell division. Cohesin is essential for sister chromatid cohesion, ensuring accurate chromosome separation in mitosis and meiosis. STAG3. specifically expressed in germ cells, is vital for meiotic cohesion and fertility. Mutations in the *STAG3* gene are linked to human reproductive disorders, including premature ovarian insufficiency (POI) and non-obstructive azoospermia (NOA), highlighting its importance in gametogenesis.
STAG3 antibodies are widely used in reproductive biology research to investigate protein expression, localization, and function in germ cells. They enable detection of STAG3 in tissues (e.g., testes, ovaries) via techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies also aid in studying cohesin dynamics during meiosis, elucidating mechanisms underlying infertility or chromosomal abnormalities.
Clinically, STAG3 antibodies may serve as diagnostic tools for genetic screening in reproductive disorders. Research using these antibodies has advanced understanding of how cohesin defects contribute to aneuploidy or gametogenesis failure. However, commercial availability and specificity of high-quality STAG3 antibodies remain critical for reliable experimental outcomes. Overall, STAG3 antibodies are pivotal in exploring both basic reproductive biology and potential therapeutic targets for infertility.
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