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产品描述：人富组氨酸糖蛋白(HRG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HRG含量。试剂盒检测范围为6.25 μg/mL-400 μg/mL，可为研究HRG在凝血机制异常、免疫调节失衡及相关疾病模型中的分子作用提供可靠工具，适用于基础科研、药物筛选及病理机制探索等非诊断性研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HRG ELISA Kit; Histidine-rich glycoprotein ELISA Kit; Histidine-proline-rich glycoprotein ELISA Kit; HPRG ELISA Kit
缩写：HRG
Uniprot No.：P04196
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 μg/mL-400 μg/mL
灵敏度：1.56 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HRG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 84-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 81-97 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of human HRG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-103 | ||||
| EDTA plasma (n=4) | 96 | 89-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected4002.6042.4972.5512.4092001.8531.8241.8391.6971000.9871.0301.0090.867500.5620.5580.5600.418250.3430.3570.3500.20812.50.2250.2360.2310.0896.250.1750.1810.1780.03600.1410.1430.142 | ||||||
货期：3-5 working days
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