首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅲ型前胶原（HPCⅢ）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清样本中的HPCⅢ含量。试剂盒检测范围为25 ng/mL-300 ng/mL，适用于体外研究领域，如通过检测动物模型或细胞培养上清中的HPCⅢ水平，探索肝纤维化发生机制、筛选抗纤维化药物活性，或用于基础医学研究中组织修复与纤维化相关疾病的病理分析。为肝病研究、药物开发及分子生物学实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
缩写：HPCⅢ
种属：Homo sapiens (Human)
样本类型：serum
检测范围：25 ng/mL-300 ng/mL
灵敏度：25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<20% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 3001.3561.3421.349 2000.9671.0230.995 1000.4660.4820.474 500.2110.1990.205 250.0500.0480.049 | ||||||
货期：3-5 working days
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