| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MANF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于MANF抗体的参考文献，涵盖基础研究及治疗应用方向：
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1. **文献名称**: *"MANF Antibody-Based Tool for Monitoring Endoplasmic Reticulum Stress in Disease Models"*
**作者**: Lindholm P. et al.
**摘要**: 该研究开发了一种高特异性兔源多克隆MANF抗体，用于检测内质网应激条件下细胞或组织中MANF蛋白的表达变化。通过Western blot和免疫荧光验证其在帕金森病小鼠模型中的有效性，证明MANF可作为内质网应激的生物标志物。
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2. **文献名称**: *"Therapeutic Potential of Anti-MANF Antibodies in Modulating Neuroinflammation"*
**作者**: Chen Y. et al.
**摘要**: 研究团队设计了一种人源化单克隆MANF抗体，通过靶向结合脑内炎症区域的MANF蛋白，增强其神经保护活性。实验显示该抗体能显著减轻多发性硬化症小鼠模型的神经炎症和髓鞘损伤。
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3. **文献名称**: *"Comparative Analysis of Commercial MANF Antibodies for Reliability in Immunohistochemistry"*
**作者**: Rodríguez-Pérez I. et al.
**摘要**: 系统评估了6种市售MANF抗体在石蜡包埋组织中的特异性，发现仅两种抗体(克隆号#A5和#3H2)能有效识别人类及啮齿类样本中的MANF，强调了抗体选择对研究结果可靠性的关键影响。
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这些文献分别从抗体开发、治疗应用及实验验证角度提供了MANF抗体的研究进展。如需更多文献或具体方向，可进一步补充关键词筛选!
MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor), also known as ARMET (arginine-rich, mutated in early tumors), is a secreted protein with cytoprotective and immunomodulatory properties. Initially identified in astrocytes, it belongs to a unique family of neurotrophic factors distinct from classical neurotrophins like GDNF or BDNF. Structurally, MANF lacks a typical receptor-binding domain but contains a conserved saposin-like domain and a disordered C-terminal region, enabling interactions with intracellular and extracellular targets. Its expression is upregulated under endoplasmic reticulum (ER) stress, where it modulates the unfolded protein response (UPR) to promote cell survival, making it critical in neurodegenerative diseases (e.g., Parkinson’s, Alzheimer’s), diabetes, and inflammation.
MANF antibodies are essential tools for studying its expression, localization, and mechanisms. They enable detection via Western blotting, immunohistochemistry, and immunofluorescence, often targeting specific epitopes in its N-terminal or C-terminal regions. Such antibodies have revealed MANF’s dual roles: intracellularly, it binds GRP78 to regulate ER stress; extracellularly, it interacts with inflammatory receptors like CDNF/MANF receptors or integrins to suppress inflammation. Research using MANF antibodies has highlighted its therapeutic potential in preclinical models of stroke, diabetes, and neurodegeneration. Additionally, cross-reactivity studies with antibodies across species (human, mouse, rat) support translational research. Challenges remain in standardizing antibodies due to MANF’s structural flexibility and tissue-specific isoforms, but advancements in monoclonal antibody design continue to refine its functional characterization.
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