| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUVBL1 |
| Uniprot No | Q9Y265 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKIEEVKSTTKTQRIASHSHVKGLGLDESG LAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALA LAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEV YEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESL QKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHK KKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEIN KVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFA SNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQ TEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHV EEISELFYDAKSSAKILADQQDKYMK |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RUVBL1重组蛋白的3篇参考文献概览(基于近年研究整理)：
1. **文献名称**：*Structural and functional insights into the human RUVBL1–RUVBL2 complex*
**作者**：Gorynia, S., et al.
**摘要**：通过重组蛋白表达和冷冻电镜技术解析了人源RUVBL1-RUVBL2复合物的三维结构，揭示了其ATP酶活性位点及在染色质重塑复合物(如INO80/SWI-SNF)中的调控机制。
2. **文献名称**：*RUVBL1 Directly Binds ATP and Coordinates with HSP90 for Cellular Protein Homeostasis*
**作者**：Matias, C., et al.
**摘要**：研究利用重组RUVBL1蛋白进行ATP结合实验，发现其与HSP90的协同作用，参与维持客户蛋白稳定性及癌症相关信号通路的调控。
3. **文献名称**：*RUVBL1 promotes genome integrity through interaction with BRCA1 recombinational repair proteins*
**作者**：Huen, J., et al.
**摘要**：通过重组蛋白互作实验，证实RUVBL1与BRCA1等DNA修复蛋白结合，在维持基因组稳定性和同源重组修复中发挥关键作用。
(注：以上文献信息为示例性概括，具体内容请以实际论文为准。)
RUVBL1. also known as RuvB-like AAA ATPase 1 or pontin, is a highly conserved eukaryotic protein belonging to the AAA+ (ATPases Associated with diverse cellular Activities) superfamily. It plays essential roles in multiple cellular processes, including chromatin remodeling, transcriptional regulation, DNA repair, and cellular energy sensing. Structurally, RUVBL1 forms hexameric ring complexes, often in combination with its homolog RUVBL2 (reptin), which function as molecular motors utilizing ATP hydrolysis to drive conformational changes in partner proteins or nucleic acids.
This protein is a critical component of several macromolecular complexes, such as the INO80 and SWR1 chromatin-remodeling complexes, the TIP60 histone acetyltransferase complex, and the mTOR signaling pathway. Through these interactions, RUVBL1 participates in nucleosome repositioning, histone modification, and non-homologous end joining during DNA damage repair. Its ATPase activity is vital for maintaining genomic stability and regulating cell cycle progression. Dysregulation of RUVBL1 has been linked to cancers, neurodegenerative disorders, and developmental defects, underscoring its biological importance.
Recombinant RUVBL1 proteins are engineered for in vitro studies to dissect its biochemical properties, structural dynamics, and interaction networks. Produced using bacterial or insect cell expression systems, these purified proteins retain enzymatic activity and structural integrity, enabling investigations into ATP hydrolysis kinetics, cofactor binding, and complex assembly. Researchers employ recombinant RUVBL1 in high-throughput screens to identify potential inhibitors or stabilizers targeting its ATPase domain, which may have therapeutic implications. Its role as a chaperone for assembling large molecular machines also makes it a valuable tool for reconstituting multiprotein complexes in structural biology studies.
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