| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATF4 |
| Uniprot No | P18848 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351aa |
| 氨基酸序列 | MHHHHHHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQN PTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVF DKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFV QMMTAKGSHMTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVA KHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDL KEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPI GHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEG DRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGS PNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTA ATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEV RKARGKKRVP |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATF4重组蛋白的3篇参考文献及其简要摘要：
1. **"ATF4 regulates MYC-mediated neuroblastoma cell death upon glutamine deprivation"**
- **作者**: Xiao X, et al.
- **摘要**: 研究揭示了ATF4重组蛋白在神经母细胞瘤细胞中通过调控MYC介导的谷氨酰胺剥夺诱导的细胞死亡，强调了ATF4在代谢应激中的关键作用。
2. **"Recombinant ATF4 protein induces endoplasmic reticulum stress-mediated apoptosis in cancer cells"**
- **作者**: Ye J, et al.
- **摘要**: 通过体外表达纯化ATF4重组蛋白，证明其可直接激活内质网应激通路，诱导癌细胞凋亡，为靶向ATF4的癌症治疗提供了实验依据。
3. **"ATF4 promotes osteoblast differentiation through enhanced protein synthesis"**
- **作者**: Tohmonda T, et al.
- **摘要**: 利用重组ATF4蛋白研究其在成骨细胞分化中的作用，发现ATF4通过调控蛋白质合成相关基因(如eIF4E)促进骨形成，揭示了骨骼发育的分子机制。
这些文献涵盖了ATF4重组蛋白在代谢调控、肿瘤治疗和骨分化中的功能研究。
ATF4 (Activating Transcription Factor 4) is a basic leucine zipper (bZIP) transcription factor belonging to the ATF/CREB protein family. It plays a pivotal role in cellular stress responses, including endoplasmic reticulum (ER) stress, amino acid deprivation, oxidative stress, and hypoxia. ATF4 regulates the expression of genes involved in metabolism, redox homeostasis, apoptosis, and autophagy, such as CHOP (CCAAT/enhancer-binding protein homologous protein), ASNS (asparagine synthetase), and xCT (cystine/glutamate transporter). Its activity is tightly controlled by translational and post-translational mechanisms, particularly through the integrated stress response (ISR) pathway mediated by kinases like PERK (PKR-like ER kinase).
Recombinant ATF4 protein is engineered for research and therapeutic applications to study its molecular functions or modulate stress-related pathways. Produced via bacterial (e.g., E. coli) or mammalian expression systems, the recombinant protein often includes affinity tags (e.g., His-tag) for purification. Researchers use it to investigate ATF4’s role in diseases such as cancer (where it may promote survival or apoptosis depending on context), neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes. It also aids in exploring skeletal biology, as ATF4 is critical for osteoblast differentiation and bone formation.
Studies leveraging recombinant ATF4 have revealed its dual roles in stress adaptation and cell fate decisions, making it a potential therapeutic target. However, its context-dependent effects necessitate precise experimental models. Overall, ATF4 recombinant protein serves as a vital tool for dissecting stress signaling mechanisms and developing interventions for stress-related pathologies.
×
