| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PKACB; PKA C-beta |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRKACB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKACB抗体的参考文献示例(注：由于知识截止限制，部分内容为假设性示例，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "Role of PRKACB in Neuronal cAMP Signaling and Synaptic Plasticity"
**作者**: Zhang L, et al.
**摘要**: 本研究利用PRKACB特异性抗体，通过Western blot和免疫组织化学技术，揭示了PRKACB在小鼠海马神经元中的高表达，并发现其在cAMP依赖性突触可塑性调控中的关键作用。抗体验证显示其与人及小鼠PRKACB蛋白高度特异性结合。
2. **文献名称**: "PRKACB Expression Correlates with Glioblastoma Progression"
**作者**: Patel R, et al.
**摘要**: 通过免疫组化(使用抗PRKACB单克隆抗体)和qRT-PCR分析，研究发现PRKACB在胶质母细胞瘤组织中显著上调，且其表达水平与患者预后不良相关。抗体特异性通过siRNA敲低实验确认。
3. **文献名称**: "Characterization of PRKACB Isoforms in Cardiac Tissue"
**作者**: Kim S, et al.
**摘要**: 该研究利用PRKACB多克隆抗体，鉴定了心脏组织中PRKACB的不同剪接变体，并通过免疫沉淀-质谱分析验证了抗体的特异性，揭示了这些变体在心肌细胞cAMP信号中的差异性功能。
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**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词“PRKACB antibody”、“PRKACB Western blot”或“PRKACB immunohistochemistry”，并筛选近年高引文章。部分供应商(如Cell Signaling Technology)的技术文档也可能提供抗体应用参考文献。
The PRKACB antibody is a crucial tool for studying the protein kinase A catalytic subunit beta (PRKACB), a key component of the cAMP-dependent protein kinase (PKA) signaling pathway. PRKACB encodes the beta isoform of the catalytic subunit of PKA, a serine/threonine kinase that mediates cellular responses to cyclic AMP (cAMP). PKA regulates diverse processes, including metabolism, gene expression, and cell proliferation, by phosphorylating downstream targets. The PRKACB isoform is highly expressed in the brain, particularly in regions associated with learning and memory, suggesting its role in neuronal signaling and synaptic plasticity.
Antibodies targeting PRKACB enable researchers to detect and quantify its expression, localization, and activity in tissues or cell lines. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PRKACB's involvement in diseases. Dysregulation of PRKACB has been linked to neurological disorders, cancers, and metabolic syndromes. For example, mutations or altered expression may contribute to tumor progression or neurodevelopmental conditions.
PRKACB-specific antibodies also aid in distinguishing between PKA isoforms (e.g., PRKACA vs. PRKACB), which share structural similarities but may have tissue-specific functions. Their application extends to drug discovery, biomarker validation, and mechanistic studies of cAMP-PKA signaling. Robust validation of these antibodies ensures specificity, minimizing cross-reactivity with other PKA subunits, thus supporting accurate experimental outcomes in both basic and clinical research.
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