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Rabbit Polyclonal SRSF8 Antibody

  • 中文名: SRSF8抗体
  • 别    名: DSM-1; SRP46; SFRS2B
货号: IPDX13783
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesDSM-1; SRP46; SFRS2B
WB Predicted band size32 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human SRSF8
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3篇关于SRSF8抗体的代表性文献信息(基于公开数据概括,非真实引用,仅作示例参考):

1. **文献名称**:*SRSF8 regulates alternative splicing in human cancer cells via direct RNA binding*

**作者**:Zhang Y et al.

**摘要**:本研究利用SRSF8抗体进行免疫共沉淀(Co-IP)和RNA pull-down实验,发现SRSF8通过结合特定mRNA前体调控肿瘤相关基因(如BCL2L1)的可变剪接,促进癌细胞存活。Western blot验证了抗体在检测内源性SRSF8蛋白中的特异性。

2. **文献名称**:*A genome-wide CRISPR screen identifies SRSF8 as a critical factor for mRNA nuclear export*

**作者**:Lee JH et al.

**摘要**:通过CRISPR筛选结合SRSF8抗体的免疫荧光染色,发现SRSF8通过招募TREX复合体介导mRNA出核。研究显示敲低SRSF8导致mRNA在核内异常滞留,抗体特异性经siRNA敲除实验验证。

3. **文献名称**:*SRSF8 deficiency disrupts pluripotency in mouse embryonic stem cells*

**作者**:Wang R et al.

**摘要**:利用SRSF8抗体进行染色质免疫沉淀测序(ChIP-seq),揭示SRSF8通过调控多能性相关转录因子(如OCT4)的pre-mRNA剪接,维持胚胎干细胞自我更新。抗体特异性通过过表达/敲除实验交叉验证。

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**说明**:以上为模拟示例,实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词 "SRSF8 antibody" + "splicing"/"RNA" 筛选近5年文献,优先选择《Nucleic Acids Research》《Cell Reports》等期刊的研究。

背景信息

The SRSF8 antibody is a tool used to detect and study the Serine/arginine-rich splicing factor 8 (SRSF8), a member of the SR protein family involved in pre-mRNA splicing. SR proteins are essential for constitutive and alternative splicing, regulating gene expression by binding to RNA motifs and mediating spliceosome assembly. SRSF8. also known as SRp46. contains an RNA recognition motif (RRM) and a C-terminal arginine/serine-rich (RS) domain, enabling interactions with other splicing factors and RNA. It plays roles in splice site selection, mRNA export, and translation regulation. Dysregulation of SRSF8 has been linked to diseases, including cancer, where aberrant splicing contributes to tumor progression. The SRSF8 antibody is widely used in research to investigate its expression, localization (primarily nuclear), and function in cellular processes like proliferation, apoptosis, and stress responses. It is validated in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, aiding studies on splicing mechanisms and disease pathways. Commercial SRSF8 antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout/knockdown controls. Understanding SRSF8's role through antibody-based assays provides insights into RNA metabolism and potential therapeutic targets.

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