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Rabbit Polyclonal ACACA Antibody

  • 中文名: ACACA抗体
  • 别    名: ACC; ACAC; ACC1; ACCA; ACACAD
货号: IPDX13768
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/300 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesACC; ACAC; ACC1; ACCA; ACACAD
WB Predicted band size265 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human ACACA
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3篇关于ACACA抗体的参考文献概览,涵盖不同研究方向的典型应用:

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1. **文献名称**:*Acetyl-CoA carboxylase alpha is essential for mammary gland development and lactation*

**作者**:Chakravarthy MV, et al.

**摘要**:研究利用ACACA特异性抗体进行Western blot及免疫组化分析,证实ACACA在小鼠乳腺发育中的关键作用,敲除后导致脂肪酸合成障碍及哺乳功能丧失。

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2. **文献名称**:*ACACA promotes tumor progression by regulating autophagy in hepatocellular carcinoma*

**作者**:Li Y, et al.

**摘要**:通过ACACA抗体进行免疫荧光和流式细胞术,发现ACACA在肝癌中高表达,通过调控自噬促进肿瘤生长,提示其作为潜在治疗靶点。

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3. **文献名称**:*Antibody-based profiling of metabolic enzymes in human adipose tissue*

**作者**:Smith SJ, et al.

**摘要**:采用多种代谢酶抗体(包括ACACA)对肥胖患者脂肪组织进行蛋白组学分析,揭示ACACA表达与胰岛素抵抗的相关性,为代谢疾病机制提供依据。

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**备注**:实际引用时建议通过PubMed/Google Scholar核对最新研究,并补充DOI及期刊信息。ACACA抗体常用于代谢、癌症及发育生物学领域,需根据实验需求(如种属、应用场景)选择已验证的抗体品牌(如Cell Signaling Technology #3676)。

背景信息

The ACACA antibody is designed to detect acetyl-CoA carboxylase alpha (ACACA), a critical enzyme in fatty acid biosynthesis. ACACA catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis, and is regulated by hormonal and nutritional signals. It exists in two isoforms (ACC1 and ACC2), with ACC1 primarily localized in the cytoplasm and involved in lipogenesis, while ACC2 is mitochondrial-associated and regulates fatty acid oxidation. Dysregulation of ACACA is linked to metabolic disorders (e.g., obesity, diabetes) and cancers, where overexpression promotes tumor growth by fueling membrane biosynthesis and energy storage. The ACACA antibody is widely used in research to study its expression, post-translational modifications (e.g., phosphorylation at Ser79), and interactions in cellular metabolism. It is validated in applications like Western blotting, immunohistochemistry, and immunofluorescence, often utilizing tissues or cell lines with high metabolic activity (e.g., liver, adipose, cancer cells). Researchers also employ this antibody to explore therapeutic targeting of ACACA in metabolic syndromes and oncology. Specificity is confirmed via knockout controls or siRNA knockdown. Commercial variants may differ in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and epitope recognition.

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