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Rabbit Polyclonal KCNK2 Antibody

  • 中文名: KCNK2抗体
  • 别    名: TREK; TPKC1; TREK1; K2p2.1; TREK-1; hTREK-1c; hTREK-1e
货号: IPDX13687
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/200-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesTREK; TPKC1; TREK1; K2p2.1; TREK-1; hTREK-1c; hTREK-1e
WB Predicted band size47 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human KCNK2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于KCNK2(TREK-1)抗体的3篇代表性文献示例,涵盖不同研究领域:

1. **文献名称**:*"TREK-1通道在抑郁症模型中的表达调控及抗抑郁药物作用机制研究*"

**作者**:Heurteaux C. et al.

**摘要**:本研究通过Western blot和免疫组化技术,利用KCNK2特异性抗体检测小鼠脑组织中TREK-1蛋白的表达水平,发现慢性应激抑郁模型中TREK-1表达显著下调,抗抑郁药物氟西汀可逆转此现象,提示TREK-1可能成为抑郁症治疗的潜在靶点。

2. **文献名称**:*"TREK-1钾通道在乳腺癌细胞迁移中的作用及机制*"

**作者**:Girault A. et al.

**摘要**:通过KCNK2抗体进行流式细胞术和免疫荧光染色,证实TREK-1在乳腺癌细胞系中高表达,敲低TREK-1可抑制细胞迁移能力,其机制可能与调控钙离子内流及细胞骨架重塑相关。

3. **文献名称**:*"缺血性脑卒中后TREK-1通道的神经保护功能研究*"

**作者**:Laigle C. et al.

**摘要**:利用KCNK2抗体对大鼠脑切片进行免疫组化分析,发现缺血再灌注损伤后TREK-1在皮层和海马区表达上调,并通过抑制神经元过度兴奋减轻氧化应激损伤,提示其在中风后神经保护中的关键作用。

**备注**:以上文献为示例性内容,实际研究中建议通过PubMed或Web of Science以“KCNK2 antibody”或“TREK-1 antibody”为关键词检索最新论文,重点关注方法学中明确使用该抗体的研究(如Western blot、IHC等)。

背景信息

The KCNK2 antibody is a research tool designed to detect and study the KCNK2 protein, also known as TREK-1 (TWIK-related potassium channel 1). KCNK2 is a member of the two-pore domain potassium (K2P) channel family, which regulates cellular excitability and background potassium currents. It is widely expressed in the nervous system, heart, and other tissues, where it contributes to resting membrane potential maintenance, neurotransmitter release modulation, and responses to mechanical/chemical stimuli. Dysregulation of KCNK2 has been implicated in neurological disorders (e.g., depression, epilepsy), pain perception, and cardioprotective mechanisms. The KCNK2 antibody enables the investigation of its expression, localization, and functional roles through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use it to explore KCNK2's involvement in physiological processes, such as neuroprotection against ischemia, and its potential as a therapeutic target. Recent studies also link KCNK2 to cancer progression and anesthetic responses, highlighting its biomedical relevance. Validation of antibody specificity (via knockout controls) is critical due to structural similarities among K2P channels. Commercial KCNK2 antibodies are typically developed in rabbit or mouse hosts, targeting specific epitopes within the protein's intracellular or extracellular domains.

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