| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | THIK2; THIK-2; K2p12.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human KCNK12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KCNK12抗体的3-4篇参考文献的简要整理：
1. **"KCNK12/TALK-1 regulates pancreatic β-cell electrical activity and insulin secretion"**
- **作者**: Dadi, P.K. et al.
- **摘要**: 研究利用KCNK12特异性抗体，通过免疫组化和Western blot验证其在胰腺β细胞的表达，揭示KCNK12通过调控膜电位影响胰岛素分泌。
2. **"Dysregulation of potassium channels in human cancers: Focus on KCNK12"**
- **作者**: Gerry, A.G. et al.
- **摘要**: 通过免疫组织化学(使用KCNK12抗体)分析肿瘤样本，发现KCNK12在结直肠癌中过表达，并与其促癌作用及患者预后不良相关。
3. **"Development of a monoclonal antibody targeting KCNK12 for functional studies"**
- **作者**: Smith, R.T. et al.
- **摘要**: 报道一种新型KCNK12单克隆抗体的开发与验证，证实其特异性可用于流式细胞术和免疫沉淀，为通道功能研究提供工具。
4. **"Potassium channels as therapeutic targets: Advances in antibody-based research"**
- **作者**: Lee, J. & Kim, S.
- **摘要**: 综述中提及KCNK12抗体在疾病机制研究中的应用，强调其在神经退行性疾病模型中的蛋白定位及表达定量分析价值。
(注：以上文献为示例，实际引用需根据具体研究内容核实。)
The KCNK12 antibody targets the KCNK12 protein, a member of the two-pore domain potassium (K2P) channel family, also known as TALK-1 (TWIK-related alkaline pH-activated K+ channel 1). KCNK12 channels are voltage-independent, pH-sensitive potassium channels primarily expressed in pancreatic β-cells and the central nervous system. They play a critical role in regulating cell membrane potential, electrical excitability, and hormone secretion. In pancreatic β-cells, KCNK12 modulates insulin secretion by influencing calcium signaling and cell excitability, making it a potential therapeutic target for diabetes research. Dysfunctional TALK-1 channels have been linked to impaired glucose homeostasis and type 2 diabetes in genetic studies.
KCNK12 antibodies are widely used in biomedical research to investigate channel expression, localization, and functional roles. Applications include Western blotting, immunohistochemistry, and immunofluorescence to study tissue-specific distribution or disease-related alterations. These antibodies are also employed in electrophysiological studies to validate channel activity when combined with genetic tools like siRNA or CRISPR. Commercially available KCNK12 antibodies vary in host species, clonal specificity (monoclonal/polyclonal), and epitope recognition, requiring validation for target-specific applications. Recent studies highlight its interaction with cellular pH changes and metabolic pathways, emphasizing its relevance in both endocrine and neurological disorders.
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