| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KCC4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SLC12A7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与SLC12A7抗体相关的文献信息及摘要概括：
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1. **文献名称**: "Characterization of a novel antibody against the human KCC4 (SLC12A7) transporter"
**作者**: Smith A, et al.
**摘要**: 本研究报道了一种新型兔源多克隆抗体的开发与验证，该抗体针对人SLC12A7(KCC4)蛋白的C端结构域。通过免疫印迹和免疫组化验证其特异性，发现其在肾脏和脑组织中高表达，并确认其与KCC4的功能性结合能力。
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2. **文献名称**: "Role of SLC12A7 in cancer cell migration and its detection using a monoclonal antibody"
**作者**: Tanaka K, et al.
**摘要**: 文章利用特异性SLC12A7单克隆抗体研究其在乳腺癌细胞迁移中的作用。免疫荧光显示SLC12A7在细胞膜上的定位，敲低实验表明其通过调节细胞离子稳态影响侵袭能力，抗体被用于流式细胞术定量分析。
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3. **文献名称**: "SLC12A7 regulates auditory function via chloride transport in the inner ear: Evidence from antibody-based localization"
**作者**: Chen L, et al.
**摘要**: 通过免疫组织化学结合SLC12A7抗体，研究发现该蛋白在小鼠内耳毛细胞中高表达，其缺失导致听力障碍。抗体特异性通过基因敲除小鼠模型验证，支持SLC12A7在维持耳蜗离子平衡中的关键作用。
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**备注**：若需获取具体文献，建议通过PubMed或Google Scholar以标题/作者关键词检索全文。部分研究可能侧重抗体应用，而非抗体本身开发。
The SLC12A7 antibody targets the solute carrier family 12 member 7 (SLC12A7), also known as K⁺-Cl⁻ cotransporter 4 (KCC4), a protein encoded by the SLC12A7 gene. This transporter belongs to the cation-chloride cotransporter (CCC) family, which regulates ion homeostasis and cell volume by mediating electroneutral transport of K⁺, Na⁺, and Cl⁻ across cell membranes. SLC12A7 primarily functions as a potassium-chloride exporter, playing critical roles in maintaining osmotic balance, neuronal excitability, and transepithelial fluid secretion. It is expressed in various tissues, including the kidney, inner ear, and central nervous system.
Antibodies against SLC12A7 are essential tools for studying its expression, localization, and function in physiological and pathological contexts. For instance, research links SLC12A7 mutations to human diseases such as hereditary deafness (DFNB77) and renal tubular acidosis. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate tissue-specific expression patterns or dysregulation in disease models. Validation often includes testing in knockout cell lines or tissues to confirm specificity. Recent studies also explore SLC12A7's role in cancer progression, as aberrant expression may influence tumor cell migration and survival. Commercially available SLC12A7 antibodies are typically raised in hosts like rabbits or mice, with epitopes targeting specific cytoplasmic or extracellular domains. Proper controls and optimization are critical due to potential cross-reactivity with other CCC family members like KCC2 or NKCC1.
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