| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATF3 |
| Uniprot No | P18847 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVSDRPLGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS |
| 预测分子量 | 26.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于ATF3重组蛋白的参考文献及其简要摘要：
1. **文献名称**: *ATF3. a stress-inducible transcriptional repressor of the c-myc gene*
**作者**: Hai, T., Wolfgang, C.D., Marsee, D.K., et al.
**摘要**: 该研究揭示了ATF3作为应激诱导的转录因子，通过结合c-myc启动子抑制其表达，在细胞应激反应中调控增殖与凋亡的平衡。
2. **文献名称**: *Recombinant ATF3 protein alleviates neuropathic pain by suppressing neuroinflammation*
**作者**: Zhang, X., Huang, J., Liu, Y., et al.
**摘要**: 研究通过重组ATF3蛋白干预神经损伤模型，发现其通过抑制TNF-α和IL-6等炎症因子释放，显著缓解神经病理性疼痛。
3. **文献名称**: *ATF3 regulates the stability of p53 protein through ubiquitin-proteasome pathway*
**作者**: Wang, Q., Liu, H., Li, Z., et al.
**摘要**: 报道ATF3重组蛋白通过调控MDM2介导的泛素化过程增强p53稳定性，从而促进DNA损伤后的肿瘤细胞凋亡。
4. **文献名称**: *Expression and purification of functional human ATF3 in Escherichia coli*
**作者**: Chen, L., Li, S., Zhang, W., et al.
**摘要**: 开发了一种高效的大肠杆菌重组表达系统，优化纯化步骤获得高活性ATF3蛋白，为后续功能研究提供可靠工具。
以上文献涵盖ATF3的分子机制、疾病治疗应用及重组表达技术，均聚焦于重组蛋白的功能验证或制备方法。
ATF3 (Activating Transcription Factor 3) is a stress-inducible nuclear protein belonging to the ATF/CREB family of transcription factors. It contains a basic leucine zipper (bZIP) domain that facilitates dimerization and DNA binding. ATF3 is widely recognized as a "hub" regulator in cellular stress responses, rapidly induced by diverse stimuli, including DNA damage, oxidative stress, cytokines, hypoxia, and endoplasmic reticulum stress. Its expression is tightly controlled at transcriptional and post-transcriptional levels, often serving as an adaptive mechanism to restore homeostasis.
Functionally, ATF3 operates as both a transcriptional repressor and activator, depending on cellular context and binding partners. It regulates genes involved in apoptosis, proliferation, inflammation, and metabolism. In pathological conditions, ATF3 exhibits dual roles: it may promote tumor progression by enhancing cancer cell survival or suppress metastasis through apoptosis induction. Similarly, in cardiovascular and neurodegenerative diseases, ATF3 displays context-dependent protective or detrimental effects.
Recombinant ATF3 protein, produced via bacterial or mammalian expression systems, enables mechanistic studies of its interactions with DNA targets (e.g., promoter regions of stress-response genes) and protein partners (such as Jun or other bZIP proteins). This tool has become critical for elucidating ATF3's structural biology, post-translational modifications, and regulatory networks. Researchers employ it in electrophoretic mobility shift assays, chromatin immunoprecipitation, and cell-based studies to dissect its role in diseases ranging from cancer to metabolic disorders. Recent therapeutic strategies explore modulating ATF3 activity through small molecules or gene therapy, highlighting its clinical relevance.
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