| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CBP; RSTS; KAT3A; RSTS1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CREBBP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与CREBBP抗体相关的模拟参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: "CREBBP Antibody Validation in Chromatin Immunoprecipitation Assays"
**作者**: Zhang L, et al.
**摘要**: 本研究验证了兔源CREBBP多克隆抗体在染色质免疫沉淀(ChIP)中的特异性，通过siRNA敲低实验和质谱分析确认其与靶蛋白的结合效率，应用于组蛋白乙酰化调控机制研究。
2. **文献名称**: "Role of CREBBP in Neural Development: Insights from Immunohistochemistry"
**作者**: Martinez R, et al.
**摘要**: 使用小鼠脑组织切片进行CREBBP抗体的免疫组化分析，揭示其在神经前体细胞分化中的时空表达模式，并发现与自闭症模型相关的乙酰化修饰异常。
3. **文献名称**: "CREBBP Antibody Application in Acute Myeloid Leukemia Biomarker Detection"
**作者**: Kim H, et al.
**摘要**: 通过Western blot和流式细胞术比较商业CREBBP抗体的灵敏度，筛选出适用于AML患者样本中低表达CREBBP蛋白检测的抗体，为临床预后评估提供工具。
(注：以上文献为示例，实际引用需检索PubMed等数据库获取真实信息。)
The CREBBP (CREB-binding protein) antibody is a crucial tool in studying the functional roles of the CREBBP protein, a transcriptional coactivator involved in regulating gene expression through histone acetylation. CREBBP, located on chromosome 16p13.3. interacts with CREB (cAMP response element-binding protein) and other transcription factors to modulate chromatin structure and enhance transcriptional activation. It contains a histone acetyltransferase (HAT) domain, enabling it to acetylate histones and non-histone proteins, thereby influencing cellular processes like cell growth, differentiation, and DNA repair. Dysregulation of CREBBP is linked to cancers (e.g., lymphomas, acute leukemia) and Rubinstein-Taybi syndrome, a developmental disorder caused by CREBBP mutations.
CREBBP antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding interactions. These antibodies help elucidate CREBBP's role in signaling pathways (e.g., Wnt, p53) and its partnership with paralog EP300. Researchers also employ CREBBP antibodies to explore its involvement in epigenetic regulation, immune responses, and therapeutic targeting in diseases. Validating antibody specificity is critical, as CREBBP shares functional domains with EP300. Overall, CREBBP antibodies are indispensable for unraveling its contributions to both normal physiology and pathology.
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