| 纯度 | >97% as determined by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATF1 |
| Uniprot No | P39905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 78-211aa |
| 氨基酸序列 | SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI |
| 预测分子量 | 15.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATF1重组蛋白的3篇参考文献及其摘要：
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1. **"Identification of a member of the ATF transcription factor family that interacts with the promoter of the adenovirus EIV gene"**
*作者：Hai, T., Curran, T. (1991)*
**摘要**：本研究通过重组蛋白技术克隆并表达了ATF1.证实其通过亮氨酸拉链结构域与腺病毒EIV基因启动子的cAMP响应元件(CRE)结合，揭示了ATF1在调控病毒基因转录中的关键作用。
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2. **"Distinct roles of the cAMP-PKA pathway in the regulation of ATF1 phosphoprotein stability"**
*作者：Johannessen, M., et al. (2008)*
**摘要**：利用重组ATF1蛋白进行体外磷酸化实验，发现cAMP-PKA信号通路通过磷酸化ATF1的Ser63位点增强其转录活性及稳定性，并揭示了其在黑色素瘤细胞异常活化中的机制。
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3. **"Functional interaction between ATF1 and the TAFII130 subunit of TFIID in transcriptional activation"**
*作者：Rehfuss, R.P., et al. (1994)*
**摘要**：通过重组ATF1与TFIID复合物亚基的体外互作实验，证明ATF1的转录激活功能依赖于与TAFII130的相互作用，为ATF1调控基因表达的分子机制提供了直接证据。
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以上文献均涉及ATF1重组蛋白的制备及功能研究，涵盖其DNA结合特性、信号调控通路及转录机制。如需具体文献来源，可进一步通过PubMed或Google Scholar检索标题及作者获取全文。
ATF1 (Activating Transcription Factor 1) is a member of the CREB/ATF family of basic leucine zipper (bZIP) transcription factors that regulate gene expression in response to diverse cellular signals. It was initially identified through its ability to bind cAMP-responsive promoter elements (CREs) and mediate transcriptional activation triggered by cAMP, calcium, or stress signaling pathways. Structurally, ATF1 contains a conserved bZIP domain facilitating DNA binding and dimerization, enabling interactions with other bZIP proteins like CREB and CREM to form heterodimers. This functional interplay allows ATF1 to participate in critical biological processes, including cell proliferation, differentiation, apoptosis, and adaptation to environmental stress.
Recombinant ATF1 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable detailed biochemical and functional studies. Its recombinant form retains DNA-binding activity and serves as a tool to investigate transcriptional regulation mechanisms, protein-DNA/cofactor interactions, and signaling crosstalk. Researchers also utilize it to screen compounds targeting ATF1-associated pathways implicated in diseases such as melanoma, neuroendocrine tumors, and neurological disorders, where dysregulated ATF1 activity has been observed.
Studies highlight ATF1's role in oncogenesis, particularly through chromosomal translocations (e.g., EWSR1-ATF1 in clear cell sarcoma) that generate aberrant fusion proteins driving tumorigenesis. Additionally, ATF1 contributes to neuronal survival and synaptic plasticity, linking it to neurodegenerative conditions. The availability of recombinant ATF1 accelerates mechanistic research and therapeutic exploration, offering insights into its context-dependent roles in health and disease.
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