| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GTF3C4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是几篇 **假设性示例** 的参考文献，供参考(实际文献需通过学术数据库检索)：
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1. **文献名称**: "GTF3C4 modulates RNA polymerase III-dependent transcription in neuroblastoma"
**作者**: Smith A, et al.
**摘要**: 研究利用GTF3C4抗体通过免疫沉淀和ChIP-seq技术，揭示了GTF3C4在神经母细胞瘤中调控tRNA和5S rRNA转录的作用，并与MYCN致癌基因存在协同表达。
2. **文献名称**: "Antibody-based profiling of GTF3C4 in viral infection responses"
**作者**: Chen B, et al.
**摘要**: 通过Western blot和免疫荧光，发现GTF3C4在H1N1流感病毒感染后表达上调，可能通过调控宿主RNA Pol III转录促进病毒复制。
3. **文献名称**: "A novel monoclonal antibody for GTF3C4 characterization in chromatin remodeling"
**作者**: Kimura T, et al.
**摘要**: 开发了一种高特异性GTF3C4单克隆抗体，验证了其在染色质免疫共沉淀(ChIP)中的有效性，并用于探索GTF3C4在染色质结构调控中的新功能。
4. **文献名称**: "GTF3C4 deficiency alters cell cycle progression in vitro"
**作者**: Garcia R, et al.
**摘要**: 使用GTF3C4抗体进行敲低实验，发现GTF3C4缺失导致HeLa细胞G1/S期阻滞，提示其通过调控非编码RNA影响细胞周期。
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**注意**：以上为模拟结构，实际文献需通过 **PubMed**、**Google Scholar** 等平台检索关键词(如“GTF3C4 antibody”、“TFIIIC subunit function”)。如需具体文献，可提供数据库访问权限进一步协助查询。
The GTF3C4 antibody targets the General Transcription Factor 3C Subunit 4 (GTF3C4), a protein component of the TFIIIC complex, which is essential for RNA polymerase III (Pol III)-mediated transcription. The TFIIIC complex binds to promoter regions of small non-coding RNAs, including tRNAs, 5S rRNA, and other Pol III-dependent transcripts, facilitating their transcription by recruiting the TFIIIB complex and positioning Pol III. GTF3C4. along with other subunits (GTF3C1-6), contributes to the structural and functional integrity of TFIIIC, with roles in DNA binding and promoter recognition.
Antibodies against GTF3C4 are primarily used in research to study Pol III transcription regulation, protein-protein interactions, and subcellular localization. They are applied in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP) to investigate GTF3C4 expression, dynamics in cellular processes (e.g., cell division, stress responses), and potential dysregulation in diseases. Aberrant Pol III activity has been linked to cancers, viral infections, and genetic disorders, making GTF3C4 a subject of interest in understanding transcriptional malfunctions. Commercial GTF3C4 antibodies are validated for specificity and reproducibility, aiding in mechanistic studies of Pol III-driven transcription and its implications in pathology.
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