首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠鸟苷酸环化酶激活剂2B（尿鸟苷素）(GUCA2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GUCA2B含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于基础研究中GUCA2B在肠道生理机制、炎症性肠病模型或药物干预实验中的动态变化分析，也可用于体外细胞模型中蛋白分泌水平的定量评估，为消化道疾病相关科研提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Guca2b ELISA Kit; Guanylate cyclase activator 2B [Cleaved into: Uroguanylin ELISA Kit; UGN)] ELISA Kit
缩写：GUCA2B
Uniprot No.：P70668
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 88-102 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 84-95 | ||||||
回收率：
| The recovery of rat GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 81-92 | |||||
| EDTA plasma (n=4) | 104 | 92-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5001.9971.9431.9701.8312501.5081.4761.4921.3531251.0020.9870.9950.85662.50.5720.5650.5690.43031.250.4280.4200.4240.28515.60.2890.2760.2830.1447.80.2040.1980.2010.06200.1410.1370.139 | |||||||
货期：3-5 working days
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