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产品描述：人鸟苷酸环化酶激活剂2B（尿鸟苷素）(GUCA2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的GUCA2B含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研领域中对胃肠疾病、肾脏生理及代谢综合征等相关机制的研究，为探究GUCA2B在体液平衡调控及相关病理过程中的分子功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GUCA2B ELISA Kit; GCAP II ELISA Kit; GCAP-II ELISA Kit; Guanylate cyclase activator 2B (uroguanylin) ELISA Kit; Guanylate cyclase activator 2B ELISA Kit; Guanylate cyclase C-activating peptide II ELISA Kit; GUC2B_HUMAN ELISA Kit; GUCA2B ELISA Kit; UGN ELISA Kit; Uroguanylin ELISA Kit
缩写：GUCA2B
Uniprot No.：Q16661
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:5 | Average % | 89 | |
| Range % | 85-91 | ||
| 1:10 | Average % | 102 | |
| Range % | 98-105 | ||
| 1:20 | Average % | 99 | |
| Range % | 93-105 | ||
| 1:40 | Average % | 95 | |
| Range % | 92-97 |
回收率：
| The recovery of human GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-96 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.1552.1752.1652.038 10001.6401.6331.6371.510 5001.0691.0541.0620.935 2500.6800.6890.6850.558 1250.4380.4240.4310.304 62.50.2660.2870.2770.150 31.250.1850.1760.1810.054 00.1290.1250.127 | ||||||
货期：3-5 working days
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