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产品描述：人谷胱甘肽S-转移酶ω-1(GSTO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GSTO1含量。GSTO1（谷胱甘肽硫转移酶ω 1）是重要的解毒酶，在氧化应激、细胞内信号传导等过程中发挥作用。研究发现其与多种疾病发生发展机制相关，如癌症、神经退行性疾病等，通过调节信号通路、代谢解毒等机制参与其中。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于体外培养细胞、动物模型组织或生物体液样本中 GSTO1 的动态表达分析；为氧化还原调控、毒理学研究及代谢性疾病机制探索提供可靠工具；在氧化损伤机制、药物代谢研究及疾病模型中具有重要科研价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA407097 ELISA Kit; AI194287 ELISA Kit; AU018802 ELISA Kit; DKFZp686H13163 ELISA Kit; EC 2.5.1.18 ELISA Kit; Glutathione S transferase omega 1 ELISA Kit; Glutathione S-transferase omega 1-1 ELISA Kit; Glutathione S-transferase omega ELISA Kit; Glutathione S-transferase omega-1 ELISA Kit; Glutathione transferase omega 1 ELISA Kit; Glutathione-dependent dehydroascorbate reductase ELISA Kit; Glutathione-S-transferase like ELISA Kit; Glutathione-S-transferase omega 1 ELISA Kit; GSTO 1-1 ELISA Kit; GSTO-1 ELISA Kit; GSTO1 1 ELISA Kit; Gsto1 ELISA Kit; GSTO1_HUMAN ELISA Kit; GSTTLp28 ELISA Kit; GSTX ELISA Kit; Gtsttl ELISA Kit; MGC94845 ELISA Kit; MMA(V) reductase ELISA Kit; Monomethylarsonic acid reductase ELISA Kit; OTTHUMP00000020429 ELISA Kit; p28 ELISA Kit; S-(Phenacyl)glutathione reductase ELISA Kit; SPG-R ELISA Kit
缩写：GSTO1
Uniprot No.：P78417
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Tags & Cell Markers
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GSTO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 83-94 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 86-97 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of human GSTO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 87-96 | |||||
| EDTA plasma (n=4) | 89 | 85-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.3042.2812.2932.117 10001.4531.3731.4131.237 5000.8880.8620.8750.699 2500.5340.5200.5270.351 1250.3950.3830.3890.213 62.50.2920.2850.2890.113 31.20.2460.2380.2420.066 00.1780.1730.176 | |||||||
货期：3-5 working days
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