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Human Gelsolin ELISA Kit

  • 中文名： 人凝溶胶蛋白(Gelsolin)ELISA Kit
货号: IPD-E08160h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人凝溶胶蛋白(Gelsolin)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GSN含量。人凝溶胶蛋白（Gelsolin）是一种广泛存在于细胞、血液和脑脊液中的多功能肌动蛋白结合蛋白，其经典功能是调节肌动蛋白丝的聚合、解聚和剪切，在细胞骨架结构重排和细胞运动中发挥重要作用。最新研究发现，Gelsolin与肿瘤和阿尔茨海默症等疾病相关，其表达量在多种癌变组织中呈下降趋势，且可能通过抑制癌细胞生长来抑制肿瘤生长和转移。试剂盒检测范围为2.74 ng/ml- 2000 ng/ml，适用于探索Gelsolin在细胞生物学中的功能机制、疾病模型中的表达变化以及作为潜在生物标志物的分析，可为神经科学、肿瘤学及免疫学等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Actin depolymerizing factor ELISA Kit; Actin-depolymerizing factor ELISA Kit; ADF ELISA Kit; AGEL ELISA Kit; Brevin ELISA Kit; DKFZp313L0718 ELISA Kit; GELS_HUMAN ELISA Kit; Gelsolin ELISA Kit; Gsn ELISA Kit
  • 缩写：
    GSN
  • Uniprot No.：
    P06396
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.74 ng/ml - 2000 ng/ml
  • 灵敏度：
    2.74 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human gelsolin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    90-103


    1:2

    Average %

    94


    Range %

    88-100


    1:4

    Average %

    90


    Range %

    86-95


    1:8

    Average %

    91


    Range %

    85-99


  • 回收率：

    The recovery of human gelsolin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    92-98


    EDTA plasma (n=4)

    98

    93-106


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.735

    2.765

    2.750

    2.665


    666.66

    1.995

    2.130

    2.063

    1.978


    222.22

    1.358

    1.334

    1.346

    1.261


    74.07

    0.747

    0.751

    0.749

    0.664


    24.69

    0.461

    0.435

    0.448

    0.363


    8.23

    0.232

    0.223

    0.228

    0.143


    2.74

    0.191

    0.195

    0.193

    0.108


    0

    0.086

    0.084

    0.085



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GSN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled GSN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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